Towards a better understanding concerning carbon to nitrogen ratio and the carbon source in aerobic granular sludge

被引:14
作者
Xavier, Jessica A. [1 ,2 ,3 ]
Santos, Sara G. S. [2 ,3 ]
Monteiro, Joana P. [2 ,3 ]
Silva, Tania F. C. V. [2 ,3 ]
da Costa, Rejane H. R. [1 ]
Vilar, Vitor J. P. [2 ,3 ]
机构
[1] Univ Fed Santa Catarina, Dept Sanit & Environm Engn, Florianopolis, SC, Brazil
[2] Univ Porto, Fac Engn, LSRE Lab Separat & React Engn, LCM Lab Catalysis & Mat, Rua Dr Roberto Frias, P-4200465 Porto, Portugal
[3] Univ Porto, Fac Engn, ALiCE Associate Lab Chem Engn, Rua Dr Roberto Frias, Rua Dr Roberto Frias, P-4200465 Porto, Portugal
来源
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING | 2023年 / 11卷 / 05期
基金
瑞典研究理事会;
关键词
Aerobic granular sludge; Low-strength wastewater; C/N ratio; Organic substrate; Metabolic activity; EXTRACELLULAR POLYMERIC SUBSTANCES; BIOLOGICAL PHOSPHORUS REMOVAL; SEQUENCING BATCH REACTOR; ACTIVATED-SLUDGE; WASTE-WATER; POPULATION-DYNAMICS; MICROBIAL COMMUNITIES; COD/N RATIO; C/N RATIO; LONG-TERM;
D O I
10.1016/j.jece.2023.110457
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
This study investigated the effects of the carbon-to-nitrogen (C/N) ratio on aerobic granular sludge (AGS) using two identical biological reactors (C/N = 5 and C/N = 10). After 84 days of operation, the reactors' organic substrate was switched from a simple synthetic carbon source (acetate and propionate) to a more complex carbon source (50% volatile fatty acids and 50% fermentable substrate). The C/N = 10 resulted in smaller, uniform granules (200 < Diameter(D)(mu m) < 500) with improved settling characteristics (sludge volumetric index - SVI5 < 30 mL gTSS L-1). The nitrogen compounds in the influent influenced the protein/polysaccharide ratio (PN/PS), with higher PS content observed under C/N = 5 and lower PS content under C/N = 10. Both C/N ratios achieved high carbon removal rates (similar to 90%), but ammonia oxidation was lower under C/N = 5 (up to 45%) due to alkalinity deficiency. Insufficient alkalinity and higher nitrogen compounds in the influent negatively affected phosphorus removal by reducing the activity of phosphorus-accumulating organisms and promoting the growth of glycogen-accumulating organisms. The complexity of the organic substrate hindered microbial activities through intermediate products from glucose and amino acid metabolic pathways. EPS-producing denitrifying organisms (Thauera and Xanthomonas) were favored in C/N = 5, while at C/N = 10, organisms associated with granular biomass stability (Zoogloea and Flavobacterium) were selected. The addition of glucose in the effluent led to a significant increase in fermentative organisms (Aeromonadaceae). This study emphasizes the crucial role of the C/N ratio and organic substrate complexity in achieving optimal performance in AGS systems..
引用
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页数:15
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