Preparation of [1′-13C]citric acid as a probe in a breath test to evaluate tricarboxylic acid cycle flux

被引：0

作者：

Mitome, Hidemichi ^{[1
]}

Takenishi, Mieko ^{[1
]}

Ono, Kumiko ^{[1
]}

Kawagoe, Naoyuki ^{[2
]}

Imai, Tsunehiko ^{[2
]}

Sasaki, Yosuke ^{[2
]}

Urita, Yoshihisa ^{[2
]}

Akira, Kazuki ^{[1
]}

机构：

[1] Matsuyama Univ, Coll Pharmaceut Sci, 4-2 Bunkyo Cho, Matsuyama, Ehime 7908578, Japan

[2] Toho Univ, Sch Med, Dept Gen Med & Emergency Med, Tokyo, Japan

来源：

JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS | 2024年 / 67卷 / 03期

关键词：

breath test; carbon-13; citric acid; stable label; TCA cycle;

D O I：

10.1002/jlcr.4083

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

[1'-C-13]Citric acid (1) was efficiently prepared from dimethyl 1,3-acetonedicarboxylate in two steps as a probe for a breath test. The synthetic method was selected because of the yield and reproducibility. Compound 1 was orally administrated to rats, and the time course of the increase of (CO2)-C-13/(CO2)-C-12 ratios (Delta(CO2)-C-13) in their breath was successfully followed, indicating the metabolism of 1. Thus, the C-13-breath test using 1 is a promising method to evaluate tricarboxylic acid (TCA) cycle flux.

引用

页码：86 / 90

页数：5