Preparation of [1′-13C]citric acid as a probe in a breath test to evaluate tricarboxylic acid cycle flux

被引：0

作者：

Mitome, Hidemichi ^{[1
]}

Takenishi, Mieko ^{[1
]}

Ono, Kumiko ^{[1
]}

Kawagoe, Naoyuki ^{[2
]}

Imai, Tsunehiko ^{[2
]}

Sasaki, Yosuke ^{[2
]}

Urita, Yoshihisa ^{[2
]}

Akira, Kazuki ^{[1
]}

机构：

[1] Matsuyama Univ, Coll Pharmaceut Sci, 4-2 Bunkyo Cho, Matsuyama, Ehime 7908578, Japan

[2] Toho Univ, Sch Med, Dept Gen Med & Emergency Med, Tokyo, Japan

来源：

JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS | 2024年 / 67卷 / 03期

关键词：

breath test; carbon-13; citric acid; stable label; TCA cycle;

D O I：

10.1002/jlcr.4083

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

[1'-C-13]Citric acid (1) was efficiently prepared from dimethyl 1,3-acetonedicarboxylate in two steps as a probe for a breath test. The synthetic method was selected because of the yield and reproducibility. Compound 1 was orally administrated to rats, and the time course of the increase of (CO2)-C-13/(CO2)-C-12 ratios (Delta(CO2)-C-13) in their breath was successfully followed, indicating the metabolism of 1. Thus, the C-13-breath test using 1 is a promising method to evaluate tricarboxylic acid (TCA) cycle flux.

引用

页码：86 / 90

页数：5

共 12 条

[1] Regulation and function of the mammalian tricarboxylic acid cycle [J].

Arnold, Paige K. ;

Finley, Lydia W. S. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2023, 299 (02)

[2]

CHANCE EM, 1983, J BIOL CHEM, V258, P3785

[3]

DUBOUDIN F, 1982, SYNTHESIS-STUTTGART, P212

[4]

HIYAMA T, 1986, SYNTHESIS-STUTTGART, P645

[5]

KAPLAN RS, 1993, J BIOL CHEM, V268, P13682

[6] Investigation of Metabolism of Exogenous Glucose at the Early Stage and Onset of Diabetes Mellitus in Otsuka Long-Evans Tokushima Fatty Rats Using [1, 2, 3-13C] Glucose Breath Tests [J].

Kawagoe, Naoyuki ;

Kano, Osamu ;

Kijima, Sho ;

Tanaka, Hideki ;

Takayanagi, Masaaki ;

Urita, Yoshihisa .

PLOS ONE, 2016, 11 (08)

[7]

Modak A. S., 2020, Breathborne Biomarkers and the Human Volatilome, P273

[8]

REETZ MT, 1982, SYNTHESIS-STUTTGART, P330

[9]

Sankararaman S., 2022, STATPEARLS INTERNET

[10] Simple and noninvasive breath test using 13C-acetic acid to evaluate gastric emptying in conscious rats and its validation by metoclopramide [J].

Uchida, M ;

Endo, N ;

Shimizu, K .

JOURNAL OF PHARMACOLOGICAL SCIENCES, 2005, 98 (04) :388-395

← 1 2 →