Chitosan modified graphene field-effect transistor biosensor for ultrasensitive procalcitonin detection

被引：18

作者：

Chen, Furong ^{[1
]}

Zhang, Ying ^{[1
]}

Wang, Mingxuan ^{[1
]}

Liu, Jinghai ^{[1
]}

Hai, Wenfeng ^{[1
]}

Liu, Yushuang ^{[1
,2
]}

机构：

[1] Inner Mongolia Minzu Univ, Nano Innovat Inst NII, Coll Chem & Mat Sci, Inner Mongolia Key Lab Carbon Nanomat, Tongliao 028000, Peoples R China

[2] Inner Mongolia Minzu Univ, Key Lab Mongolian Med Res & Dev Engn, Minist Educ, Tongliao 028000, Peoples R China

来源：

TALANTA | 2024年 / 268卷

基金：

中国国家自然科学基金;

关键词：

Graphene field-effect transistor; Chitosan; Sepsis; Procalcitonin; INFLAMMATION; ASSAY;

D O I：

10.1016/j.talanta.2023.125308

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Sepsis is a systemic inflammatory response caused by a bacterial infection that often leading to tissue damage, organ failure and death. Procalcitonin (PCT), as a peptide precursor to hormones, is the main biomarker to identification of the sepsis. In this study, a chitosan modified graphene field transistor (CTS-GFET) was established and first time used for PCT ultra-sensitive detection. CTS was functionalized on the GFET channel surface to immobilized anti-PCT by glutaraldehyde. This biosensor exhibited the detection limit as low as 0.82 ag/mL in PBS, which exhibited 3 times enhancement compared with GFET biosensors. The enhancement mechanisms of CTS-GFET were studied by electrical theoretical model. In addition, the CTS-GFET biosensor was successfully applied to quantify the concentration of the PCT in human serum samples, indicating the potential use in clinical application.

引用

页数：6

共 33 条

[1] Discrimination of single-point mutations in unamplified genomic DNA via Cas9 immobilized on a graphene field-effect transistor [J].

Balderston, Sarah ;

Taulbee, Jeffrey J. ;

Celaya, Elizabeth ;

Fung, Kandace ;

Jiao, Amanda ;

Smith, Kasey ;

Hajian, Reza ;

Gasiunas, Giedrius ;

Kutanovas, Simonas ;

Kim, Daehwan ;

Parkinson, Jonathan ;

Dickerson, Kenneth ;

Ripoll, Juan-Jose ;

Peytavi, Regis ;

Lu, Hsiang-Wei ;

Barron, Francie ;

Goldsmith, Brett R. ;

Collins, Philip G. ;

Conboy, Irina M. ;

Siksnys, Virginijus ;

Aran, Kiana .

NATURE BIOMEDICAL ENGINEERING, 2021, 5 (07) :713-725

[2] Detection of canine and equine procalcitonin for sepsis diagnosis in veterinary clinic by the development of novel MIP-based SPR biosensors [J].

Battaglia, F. ;

Baldoneschi, V ;

Meucci, V ;

Intorre, L. ;

Minunni, M. ;

Scarano, S. .

TALANTA, 2021, 230

[3] Clinical review 167 -: Procalcitonin and the calcitonin gene family of peptides in inflammation, infection, and sepsis:: A journey from calcitonin back to its precursors [J].

Becker, KL ;

Nylén, ES ;

White, JC ;

Müller, B ;

Snider, RH .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2004, 89 (04) :1512-1525

[4] Pauli-limit violation and re-entrant superconductivity in moire graphene [J].

Cao, Yuan ;

Park, Jeong Min ;

Watanabe, Kenji ;

Taniguchi, Takashi ;

Jarillo-Herrero, Pablo .

NATURE, 2021, 595 (7868) :526-+

[5] NiCoP/g-C3N4 Nanocomposites-Based Electrochemical Immunosensor for Sensitive Detection of Procalcitonin [J].

Chen, Furong ;

Bao, Layue ;

Zhang, Ying ;

Wang, Ruili ;

Liu, Jinghai ;

Hai, Wenfeng ;

Liu, Yushuang .

SENSORS, 2023, 23 (09)

[6] Early sepsis diagnosis via protein and miRNA biomarkers using a novel point-of-care photonic biosensor [J].

Fabri-Faja, Nuria ;

Calvo-Lozano, Olalla ;

Dey, Priyanka ;

Terborg, Roland A. ;

Estevez, M-Carmen ;

Belushkin, Alexander ;

Yesilkoy, Filiz ;

Duempelmann, Luc ;

Altug, Hatice ;

Pruneri, Valerio ;

Lechuga, Laura M. .

ANALYTICA CHIMICA ACTA, 2019, 1077 :232-242

[7] Poly-L-Lysine-Modified Graphene Field-Effect Transistor Biosensors for Ultrasensitive Breast Cancer miRNAs and SARS-CoV-2 RNA Detection [J].

Gao, Jianwei ;

Wang, Chunhua ;

Wang, Chao ;

Chu, Yujin ;

Wang, Shun ;

Sun, Ming Yuan ;

Ji, Hao ;

Gao, Yakun ;

Wang, Yanhao ;

Han, Yingkuan ;

Song, Fangteng ;

Liu, Hong ;

Zhang, Yu ;

Han, Lin .

ANALYTICAL CHEMISTRY, 2022, 94 (03) :1626-1636

[8] Label-free electrochemical biosensor for determination of procalcitonin based on graphene-wrapped Co nanoparticles encapsulated in carbon nanobrushes coupled with AuPtCu nanodendrites [J].

Ge, Xin-Yue ;

Zhang, Jing-Xian ;

Feng, Yi-Ge ;

Wang, Ai-Jun ;

Mei, Li-Ping ;

Feng, Jiu-Ju .

MICROCHIMICA ACTA, 2022, 189 (03)

[9] The rise of graphene [J].

Geim, A. K. ;

Novoselov, K. S. .

NATURE MATERIALS, 2007, 6 (03) :183-191

[10] Comparison of CRP and procalcitonin for etiological diagnosis of fever during febrile neutropenia in hematology patients- an experience from a tertiary care center in Northern India [J].

Halder, Rohan ;

Seth, Tulika ;

Chaturvedi, Pradeep K. ;

Mishra, Priyanka ;

Mahapatra, Manoranjan ;

Pati, Hara P. ;

Tyagi, Seema ;

Saxena, Renu .

BLOOD CELLS MOLECULES AND DISEASES, 2020, 84

← 1 2 3 4 →