Oral delivery of a highly stable superoxide dismutase as a skin aging inhibitor

被引：6

作者：

Dong, Liang ^{[1
,5
]}

Chen, Yongli ^{[1
,2
]}

Gu, Lihong ^{[1
]}

Gan, Miao ^{[1
]}

Carrier, Andrew ^{[3
]}

Oakes, Ken ^{[4
]}

Zhang, Xu ^{[3
]}

Dong, Zhiyang ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, POB 2714, Beijing 100080, Peoples R China

[2] Shenzhen Siyomicro Biotech CO Ltd, Shenzhen 518116, Peoples R China

[3] Cape Breton Univ, Dept Chem, 1250 Grand Lake Rd, Sydney, NS B1P 6L2, Canada

[4] Cape Breton Univ, Dept Biol, 1250 Grand Lake Rd, Sydney, NS B1P 6L2, Canada

[5] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

BIOMEDICINE & PHARMACOTHERAPY | 2023年 / 164卷

基金：

加拿大自然科学与工程研究理事会;

关键词：

Skin aging; Superoxide dismutase; Oral delivery; Antioxidant; Bioavailability; ANTIINFLAMMATORY PROPERTIES; TARGETED DELIVERY; D-GALACTOSE; ANTIOXIDANT; SUPPLEMENTATION; EXPRESSION; PROTECTS; MODEL; MICE;

D O I：

10.1016/j.biopha.2023.114878

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

As an effective antioxidant enzyme, superoxide dismutase (SOD) has been widely used as a food supplement, cosmetic additive, and therapeutic agent. However, oral delivery of SOD is challenging due to its relative instability, limited bioavailability, and low absorption efficiency in the gastrointestinal (GI) tract. We addressed these issues using a highly stable superoxide dismutase (hsSOD) generated from a hot spring microbial sample. This SOD exhibited a specific activity of 5000 IU/mg while retaining its enzymatic activity under low pH en-vironments of an artificial GI system and in the presence of surfactants and various proteolytic enzymes. The inhibitory effects of hsSOD against skin-aging was evaluated under both in vitro and in vivo experiments using fibroblast cell and D-galactose induced aging-mouse models, respectively. Effective oral delivery of hsSOD promises wide applicability in pharmaceutical and food industries.

引用

页数：11

共 77 条

[1] Antioxidant enzymes and lipid peroxidation in the scalp of patients with alopecia areata [J].

Akar, A ;

Arca, E ;

Erbil, HA ;

Akay, C ;

Sayal, A ;

Gür, AR .

JOURNAL OF DERMATOLOGICAL SCIENCE, 2002, 29 (02) :85-90

[2] Copper/Zinc Superoxide Dismutase in Human Skin: Current Knowledge [J].

Altobelli, Giovanna G. ;

Van Noorden, Susan ;

Balato, Anna ;

Cimini, Vincenzo .

FRONTIERS IN MEDICINE, 2020, 7

[3] ASPECTS OF THE STRUCTURE, FUNCTION, AND APPLICATIONS OF SUPEROXIDE-DISMUTASE [J].

BANNISTER, JV ;

BANNISTER, WH ;

ROTILIO, G .

CRC CRITICAL REVIEWS IN BIOCHEMISTRY, 1987, 22 (02) :111-180

[4]

BARTSCH G, 1981, EUR J RHEUMATOL INFL, V4, P250

[5] SOD Therapeutics: Latest Insights into Their Structure-Activity Relationships and Impact on the Cellular Redox-Based Signaling Pathways [J].

Batinic-Haberle, Ines ;

Tovmasyan, Artak ;

Roberts, Emily R. H. ;

Vujaskovic, Zeljko ;

Leong, Kam W. ;

Spasojevic, Ivan .

ANTIOXIDANTS & REDOX SIGNALING, 2014, 20 (15) :2372-2415

[6] Bio-friendly encapsulation of superoxide dismutase into vaterite CaCO3 crystals. Enzyme activity, release mechanism, and perspectives for ophthalmology [J].

Binevski, Petr V. ;

Balabushevich, Nadezhda G. ;

Uvarova, Viktoria I. ;

Vikulina, Anna S. ;

Volodkin, Dmitry .

COLLOIDS AND SURFACES B-BIOINTERFACES, 2019, 181 :437-449

[7] MANGANESE SCHIFFS BASE COMPLEXES .2. SYNTHESIS AND SPECTROSCOPY OF CHLORO-COMPLEXES OF SOME DERIVATIVES OF (SALICYLALDEHYDEETHYLENEDIIMATO) MANGANESE(III) [J].

BOUCHER, LJ .

JOURNAL OF INORGANIC & NUCLEAR CHEMISTRY, 1974, 36 (03) :531-536

[8] INFOGEST static in vitro simulation of gastrointestinal food digestion [J].

Brodkorb, Andre ;

Egger, Lotti ;

Alminger, Marie ;

Alvito, Paula ;

Assuncao, Ricardo ;

Ballance, Simon ;

Bohn, Torsten ;

Bourlieu-Lacanal, Claire ;

Boutrou, Rachel ;

Carriere, Frederic ;

Clemente, Alfonso ;

Corredig, Milena ;

Dupont, Didier ;

Dufour, Claire ;

Edwards, Cathrina ;

Golding, Matt ;

Karakaya, Sibel ;

Kirkhus, Bente ;

Le Feunteun, Steven ;

Lesmes, Uri ;

Macierzanka, Adam ;

Mackie, Alan R. ;

Martins, Carla ;

Marze, Sebastien ;

McClements, David Julian ;

Menard, Olivia ;

Minekus, Mans ;

Portmann, Reto ;

Santos, Claudia N. ;

Souchon, Isabelle ;

Singh, R. Paul ;

Vegarud, Gerd E. ;

Wickham, Martin S. J. ;

Weitschies, Werner ;

Recio, Isidra .

NATURE PROTOCOLS, 2019, 14 (04) :991-1014

[9] Superoxide Dismutase Administration, A Potential Therapy Against Oxidative Stress Related Diseases: Several Routes of Supplementation and Proposal of an Original Mechanism of Action [J].

Carillon, Julie ;

Rouanet, Jean-Max ;

Cristol, Jean-Paul ;

Brion, Richard .

PHARMACEUTICAL RESEARCH, 2013, 30 (11) :2718-2728

[10] Foundations of gastrointestinal-based drug delivery and future developments [J].

Chu, Jacqueline N. ;

Traverso, Giovanni .

NATURE REVIEWS GASTROENTEROLOGY & HEPATOLOGY, 2022, 19 (04) :219-238

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