The extraction and identification of active components of the sex pheromones of Asian citrus psyllid, Diaphorina citri

被引:4
作者
Luo, Haiyu [1 ,2 ,3 ]
Tang, Xia'an [1 ,2 ,3 ]
Deng, Yecheng [1 ,2 ,3 ,4 ]
Deng, Zhiyong [1 ,2 ,3 ]
Liu, Mingxi [1 ,2 ,3 ]
机构
[1] Guangxi Normal Univ, Key Lab Ecol Rare & Endangered Species & Environm, Guangxi Key Lab Landscape Resources Conservat & Su, Minist Educ, Guilin 541006, Guangxi, Peoples R China
[2] Guangxi Normal Univ, Guangxi Key Lab Rare & Endangered Anim Ecol, Guilin 541006, Guangxi, Peoples R China
[3] Guangxi Normal Univ, Inst Sustainable Dev & Innovat, Guilin 541006, Guangxi, Peoples R China
[4] Guangxi Normal Univ, Yanshan Campus,1 Yanzhong Rd, Guilin 541006, Peoples R China
关键词
Mating rhythm; Y-tube olfactometer; Attractant activity; Solvent extraction; Solid phase microextraction (SPME); Gas chromatography-mass spectrometry (GC-MS); CACOPSYLLA-PYRICOLA HOMOPTERA; KUWAYAMA HEMIPTERA PSYLLIDAE; INSECTICIDE RESISTANCE; POPULATIONS; MANAGEMENT;
D O I
10.1016/j.pestbp.2023.105421
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this study, we extracted and identified the active components of the Asian citrus psyllid, Diaphorina citri sex pheromones to provide a basis for further development of sex attractants. Under laboratory conditions, mating activity in D. citri started 3 d after emergence, which peaked at 6-7 d, and mating activity had no obvious peak during the observed period 7:00-21:00 h. Additionally, D. citri males were attracted to the emanations from conspecific females, especially to the n-hexane extracts of the pheromone. A total of 17 compounds were identified from the n-hexane extracts of female and male D. citri by gas chromatography-mass spectrometer (GC-MS). Among them, 13 compounds were identified from the female D. citri n-hexane extracts, of which 7 (dichloromethane, acetic acid, toluene, butyl acetate, ethyl carbamoylacetate, alpha-pinene, and 1-nonanal) were not found in the male D. citri n-hexane extracts. In addition, a total of 33 compounds were identified from the solid phase microextraction (SPME) volatiles of the male and female D. citri adults. Among these, 17 compounds were identified from the female D. citri volatiles, of which 6 (cycloheptatriene, 5-methyl-2-phenylindole, 1-dodecanol, cis-11-hexadecena, dodecyl aldehyde, and nerylacetone) were not identified in the volatiles of the D. citri males. It was found that males were significantly attracted to 0.1-10 mu L/mL acetic acid and 1-nonanal with the selection rates ranging from 62.04%-70.56% and 62.22%-67.22%, respectively. Therefore, the results of this study suggest that acetic acid and 1-nonanal might be the active compounds of the female D. citri sex pheromones.
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页数:9
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