Beneficial Effects of Citrus Bergamia Polyphenolic Fraction on Saline Load-Induced Injury in Primary Cerebral Endothelial Cells from the Stroke-Prone Spontaneously Hypertensive Rat Model

被引:3
作者
Stanzione, Rosita [1 ]
Forte, Maurizio [1 ]
Cotugno, Maria [1 ]
Oppedisano, Francesca [2 ]
Carresi, Cristina [2 ]
Marchitti, Simona [1 ]
Mollace, Vincenzo [2 ,3 ]
Volpe, Massimo [3 ,4 ]
Rubattu, Speranza [1 ,4 ]
机构
[1] IRCCS Neuromed, I-86077 Pozzilli, Italy
[2] Magna Graecia Univ Catanzaro, Inst Res Food Safety & Hlth IRC FSH, Dept Hlth Sci, I-88100 Catanzaro, Italy
[3] IRCCS San Raffaele, I-00163 Rome, Italy
[4] Sapienza Univ Rome, Sch Med & Psychol, Dept Clin & Mol Med, I-00185 Rome, Italy
关键词
salt loading; endothelial cell; oxidative stress; mitochondrial dysfunction; bergamot; polyphenols; stroke; SHRSP; RENAL DAMAGE; EXPRESSION; DYSFUNCTION; EXTRACT;
D O I
10.3390/nu15061334
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
High salt load is a known noxious stimulus for vascular cells and a risk factor for cardiovascular diseases in both animal models and humans. The stroke-prone spontaneously hypertensive rat (SHRSP) accelerates stroke predisposition upon high-salt dietary feeding. We previously demonstrated that high salt load causes severe injury in primary cerebral endothelial cells isolated from SHRSP. This cellular model offers a unique opportunity to test the impact of substances toward the mechanisms underlying high-salt-induced vascular damage. We tested the effects of a bergamot polyphenolic fraction (BPF) on high-salt-induced injury in SHRSP cerebral endothelial cells. Cells were exposed to 20 mM NaCl for 72 h either in the absence or the presence of BPF. As a result, we confirmed that high salt load increased cellular ROS level, reduced viability, impaired angiogenesis, and caused mitochondrial dysfunction with a significant increase in mitochondrial oxidative stress. The addition of BPF reduced oxidative stress, rescued cell viability and angiogenesis, and recovered mitochondrial function with a significant decrease in mitochondrial oxidative stress. In conclusion, BPF counteracts the key molecular mechanisms underlying high-salt-induced endothelial cell damage. This natural antioxidant substance may represent a valuable adjuvant to treat vascular disorders.
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页数:12
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