Protocol for 3D virtual histology of unstained human brain tissue using synchrotron radiation phase-contrast microtomography

被引：5

作者：

Lee, Ju Young ^{[1
,2
]}

Donato, Sandro ^{[3
,4
,5
]}

Mack, Andreas F. ^{[6
]}

Mattheus, Ulrich ^{[6
]}

Tromba, Giuliana ^{[7
]}

Longo, Elena ^{[7
]}

D'Amico, Lorenzo ^{[7
,8
]}

Mueller, Sebastian ^{[2
]}

Shiozawa, Thomas ^{[6
]}

Bause, Jonas ^{[2
]}

Scheffler, Klaus ^{[2
,9
]}

Longo, Renata ^{[8
,10
]}

Hagberg, Gisela E. ^{[2
,9
]}

机构：

[1] Eberhard Karls Univ Tubingen, Grad Training Ctr Neurosci, Tubingen, Germany

[2] Max Planck Inst Biol Cybernet, High Field Magnet Resonance, Tubingen, Germany

[3] Univ Calabria, Dept Phys, Arcavacata Di Rende, Italy

[4] Univ Calabria, STAR LAB, Arcavacata Di Rende, Italy

[5] Ist Nazl Fis Nucl INFN, Div Frascati, Frascati, Italy

[6] Eberhard Karls Univ Tubingen, Inst Clin Anat & Cell Anal, Tubingen, Germany

[7] Elettra Sincrotrone Trieste SCpA, Basovizza, Italy

[8] Univ Trieste, Dept Phys, Trieste, Italy

[9] Univ Hosp Tubingen, Dept Biomed Magnet Resonance, Tubingen, Germany

[10] Ist Nazl Fis Nucl INFN, Div Trieste, Trieste, Italy

来源：

FRONTIERS IN PHYSICS | 2024年 / 11卷

关键词：

X-ray phase-contrast microtomography; virtual histology; synchrotron radiation X-ray; soft-tissue imaging; 3D imaging; COMPUTED-TOMOGRAPHY; MAGNETIC-RESONANCE; CORPORA-AMYLACEA; IMAGE; CT; MORPHOLOGY; FIXATION; PLATFORM; MRI;

D O I：

10.3389/fphy.2023.1335285

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

X-ray phase-contrast micro computed tomography using synchrotron radiation (SR PhC-mu CT) offers unique 3D imaging capabilities for visualizing microstructure of the human brain. Its applicability for unstained soft tissue is an area of active research. Acquiring images from a tissue block without needing to section it into thin slices, as required in routine histology, allows for investigating the microstructure in its natural 3D space. This paper presents a detailed step-by-step guideline for imaging unstained human brain tissue at resolutions of a few micrometers with SR PhC-mu CT implemented at SYRMEP, the hard X-ray imaging beamline of Elettra, the Italian synchrotron facility. We present examples of how blood vessels and neurons appear in the images acquired with isotropic 5 mu m and 1 mu m voxel sizes. Furthermore, the proposed protocol can be used to investigate important biological substrates such as neuromelanin or corpora amylacea. Their spatial distribution can be studied using specifically tailored segmentation tools that are validated by classical histology methods. In conclusion, SR PhC-mu CT using the proposed protocols, including data acquisition and image processing, offers viable means of obtaining information about the anatomy of the human brain at the cellular level in 3D.

引用

页数：18

共 91 条

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