Potential value of a rapid syndromic multiplex PCR for the diagnosis of native and prosthetic joint infections: a real-world evidence study

被引：4

作者：

Pascual, Stephanie ^{[1
]}

Noble, Brooklyn ^{[2
]}

Ahmad-Saeed, Nusreen ^{[3
]}

Aldridge, Catherine ^{[4
]}

Ambretti, Simone ^{[5
]}

Amit, Sharon ^{[6
]}

Annett, Rachel ^{[7
]}

O'Shea, Shaan Ashk ^{[3
]}

Barbui, Anna Maria ^{[8
]}

Barlow, Gavin ^{[9
]}

Barrett, Lucinda ^{[10
]}

Berth, Mario ^{[11
]}

Bondi, Alessandro ^{[12
]}

Boran, Nicola ^{[13
]}

Boyd, Sara E. ^{[14
]}

Chaves, Catarina ^{[15
]}

Clauss, Martin ^{[16
]}

Davies, Peter ^{[17
]}

Dianzo-Delgado, Ileana T. ^{[18
]}

Esteban, Jaime ^{[19
]}

Fuchs, Stefan ^{[20
]}

Friis-Hansen, Lennart ^{[21
,22
]}

Goldenberger, Daniel ^{[16
]}

Glaser, Andrej Krasevac ^{[23
]}

Groonroos, Juha O. ^{[24
]}

Hoffmann, Ines ^{[25
]}

Hoffmann, Tomer ^{[6
]}

Hughes, Harriet ^{[7
]}

Ivanova, Marina ^{[26
]}

Jezek, Peter ^{[27
]}

Jones, Gwennan ^{[7
]}

Karahan, Zeynep Ceren ^{[28
]}

Lass-Floerl, Cornelia ^{[20
]}

Laurent, Frederic ^{[29
]}

Leach, Laura ^{[10
]}

Pedersen, Matilde Lee Horsboll ^{[21
,22
]}

Loiez, Caroline ^{[30
]}

Lynch, Maureen ^{[13
]}

Maloney, Robert J. ^{[9
]}

Marsh, Martin ^{[4
]}

Milburn, Olivia ^{[4
]}

Mitchell, Shanine ^{[7
]}

Moore, Luke S. P. ^{[14
]}

Moffat, Lynn ^{[17
]}

Murdjeva, Marianna ^{[31
]}

Murphy, Michael E. ^{[17
]}

Nayar, Deepa ^{[4
]}

Nigrisoli, Giacomo ^{[5
]}

O'Sullivan, Fionnuala ^{[13
]}

Oez, Buesra ^{[28
]}

机构：

[1] Biomerieux, Marcy Letoile, France

[2] BioMerieux, Salt Lake City, UT USA

[3] Univ Hosp Southampton NHS Fdn Trust, Southampton, England

[4] Newcastle Tyne Hosp NHS Fdn Trust, Newcastle Upon Tyne, England

[5] IRCCS Azienda Osped Univ Bologna, S Orsola Bologna, Bologna, Italy

[6] Sheba Med Ctr, Ramat Gan, Israel

[7] Univ Hosp Wales, Cardiff, Wales

[8] Citta Salute & Sci, Dept Publ Hlth & Pediat Microbiol, Virol Unit, San Giovanni Battista, Turin, Italy

[9] Hull Univ Teaching Hosp NHS Trust, Kingston Upon Hull, England

[10] Oxford Univ Hosp OUH, Oxford, England

[11] AZ Alma, Eeklo, Belgium

[12] Univ Turin, Dept Publ Hlth & Pediat, Turin, Italy

[13] Mater Misericordiae Univ Hosp, Dublin, Ireland

[14] Chelsea & Westminster NHS Fdn Trust, London, England

[15] Ctr Hosp & Univ Coimbra, Coimbra, Portugal

[16] Univ Hosp Basel, Basel, Switzerland

[17] Univ Glasgow, Glasgow Royal Infirm, NHS Greater Glasgow & Clyde, Glasgow, Scotland

[18] Inst Ramon & Cajal Invest Sanitaria IRYCIS, Hosp Univ Ramon & Cajal, Serv Microbiol, Madrid, Spain

[19] CIBERINFEC CIBER Enfermedades Infecciosas, IIS Fdn Jimenez Diaz, Dept Clin Microbiol, Madrid, Spain

[20] Med Univ Innsbruck, Inst Hyg & Med Microbiol, Innsbruck, Austria

[21] Copenhagen Univ Hosp, Bispebjerg, Copenhagen, Denmark

[22] Rigshospitalet, Dept Clin Microbiol, Copenhagen, Denmark

[23] NZOLH Maribor, Maribor, Slovenia

[24] Varsinais Suomen Sairaanhoitopiiri, Loimaa, Finland

[25] MVZ Lab Dr Reising Ackermann & Kollegen, Leipzig, Germany

[26] East Tallinn Cent Hosp, Tallinn, Estonia

[27] Reg Hosp Pribram, Pribram, Czech Republic

[28] Ankara Univ, Sch Med, Dept Med Microbiol, Ankara, Turkiye

[29] Hosp Civils Lyon, Lyon, France

[30] Ctr Hosp Univ Lille, Lille, France

[31] Univ Hosp St George, Plovdiv, Bulgaria

[32] Hampshire Hosp NHS Fdn Trust, Winchester, England

[33] Krankenhaus Gottlicher Heiland, Vienna, Austria

[34] Orthoped Hosp Valdoltra, Koper lab, Valdoltra, Slovenia

[35] Careggi Univ Hosp, Florence, Italy

[36] Hop Ambroise Pare, APHP, Boulogne Billancourt, France

[37] Hosp Univ Ramon & Cajal, Serv Microbiol, CIBER Enfermedades Infecciosas, Inst Salud Carlos 3, Madrid, Spain

[38] Inst Ramon & Cajal Invest Sanitaria IRYCIS, Madrid, Spain

[39] Hosp Univ Marques Valdecilla, Santander, Spain

[40] Allgemeines Krankenhaus Wien, A-1090 Vienna, Austria

[41] Univ Med Ctr Groningen, Groningen, Netherlands

来源：

JOURNAL OF BONE AND JOINT INFECTION | 2024年 / 9卷 / 01期

关键词：

SEPTIC ARTHRITIS; CLINICAL-FEATURES; PROFILE;

D O I：

10.5194/jbji-9-87-2024

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Introduction: The BIOFIRE Joint Infection (JI) Panel is a diagnostic tool that uses multiplex-PCR testing to detect microorganisms in synovial fluid specimens from patients suspected of having septic arthritis (SA) on native joints or prosthetic joint infections (PJIs). Methods: A study was conducted across 34 clinical sites in 19 European and Middle Eastern countries from March 2021 to June 2022 to assess the effectiveness of the BIOFIRE JI Panel. Results: A total of 1527 samples were collected from patients suspected of SA or PJI, with an overall agreement of 88.4 % and 85 % respectively between the JI Panel and synovial fluid cultures (SFCs). The JI Panel detected more positive samples and microorganisms than SFC, with a notable difference on Staphylococcus aureus, Streptococcus species, Enterococcus faecalis, Kingella kingae, Neisseria gonorrhoeae, and anaerobic bacteria. The study found that the BIOFIRE JI Panel has a high utility in the real-world clinical setting for suspected SA and PJI, providing diagnostic results in approximately 1 h. The user experience was positive, implying a potential benefit of rapidity of results' turnover in optimising patient management strategies. Conclusion: The study suggests that the BIOFIRE JI Panel could potentially optimise patient management and antimicrobial therapy, thus highlighting its importance in the clinical setting.

引用

页码：87 / 97

页数：11