Durable 3D murine ex vivo retina glaucoma models for optical coherence tomography

被引:3
作者
Barroso, Alvaro [1 ]
Ketelhut, Steffi [1 ]
Nettels-Hackert, Gerburg [2 ]
Heiduschka, Peter [2 ]
Del Amor, Rocio [3 ]
Naranjo, Valery [3 ]
Kemper, Bjorn [1 ]
Schnekenburger, Jurgen [1 ]
机构
[1] Univ Munster, Fac Med, Biomed Technol Ctr, Mendelstr 17, D-48149 Munster, Germany
[2] Univ Munster, Fac Med, Dept Ophthalmol, Domagkstr 15, D-48149 Munster, Germany
[3] Univ Politecn Valencia, Inst Univ Invest Tecnol Centrada el Ser Humano, Valencia, Spain
基金
欧盟地平线“2020”;
关键词
FIBER LAYER THICKNESS; GANGLION-CELLS; PHANTOM EYE; QUALITY ASSESSMENT; DEGENERATION; RESOLUTION; GENE; IDENTIFICATION; DEATH;
D O I
10.1364/BOE.494271
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Durable and standardized phantoms with optical properties similar to native healthy and disease-like biological tissues are essential tools for the development, performance testing, calibration and comparison of label-free high-resolution optical coherence tomography (HR-OCT) systems. Available phantoms are based on artificial materials and reflect thus only partially ocular properties. To address this limitation, we have performed investigations on the establishment of durable tissue phantoms from ex vivo mouse retina for enhanced reproduction of in vivo structure and complexity. In a proof-of-concept study, we explored the establishment of durable 3D models from dissected mouse eyes that reproduce the properties of normal retina structures and tissue with glaucoma-like layer thickness alterations. We explored different sectioning and preparation procedures for embedding normal and N-methyl-D-aspartate (NMDA)-treated mouse retina in transparent gel matrices and epoxy resins, to generate durable three-dimensional tissue models. Sample quality and reproducibility were quantified by thickness determination of the generated layered structures utilizing computer-assisted segmentation of OCT B-scans that were acquired with a commercial HR-OCT system at a central wavelength of 905 nm and analyzed with custom build software. Our results show that the generated 3D models feature thin biological layers close to current OCT resolution limits and glaucoma-like tissue alterations that are suitable for reliable HR-OCT performance characterization. The comparison of data from resin-embedded tissue with native murine retina in gels demonstrates that by utilization of appropriate preparation protocols, highly stable samples with layered structures equivalent to native tissues can be fabricated. The experimental data demonstrate our concept as a promising approach toward the fabrication of durable biological 3D models suitable for high-resolution OCT system performance characterization supporting the development of optimized instruments for ophthalmology applications.
引用
收藏
页码:4421 / 4438
页数:18
相关论文
共 74 条
  • [1] Optic Nerve Head Measurements With Optical Coherence Tomography: A Phantom-Based Study Reveals Differences Among Clinical Devices
    Agrawal, Anant
    Baxi, Jigesh
    Calhoun, William
    Chen, Chieh-Li
    Ishikawa, Hiroshi
    Schuman, Joel S.
    Wollstein, Gadi
    Hammer, Daniel X.
    [J]. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2016, 57 (09) : OCT413 - OCT420
  • [2] Morphology and spatial refractive index distribution of the retina accessed by hyperspectral quantitative phase microscopy
    Barroso, Alvaro
    Ketelhut, Steffi
    Heiduschka, Peter
    Nettels-Hackert, Gerburg
    Schnekenburger, Juergen
    Kemper, Bjoern
    [J]. OPTICAL METHODS FOR INSPECTION, CHARACTERIZATION, AND IMAGING OF BIOMATERIALS IV, 2019, 11060
  • [3] Refractive index determination of buffer solutions from visible to near-infrared spectral range for multispectral quantitative phase imaging using a calibrated Abbe refractometer
    Barroso, Alvaro
    Radhakrishnan, Rohan
    Ketelhut, Steffi
    Schnekenburger, Juergen
    Kemper, Bjoern
    [J]. QUANTITATIVE PHASE IMAGING V, 2019, 10887
  • [4] Retina-simulating phantom for optical coherence tomography
    Baxi, Jigesh
    Calhoun, William
    Sepah, Yasir Jamal
    Hammer, Daniel X.
    Ilev, Ilko
    Pfefer, T. Joshua
    Quan Dong Nguyen
    Agrawal, Anant
    [J]. JOURNAL OF BIOMEDICAL OPTICS, 2014, 19 (02)
  • [5] Animal Models of Glaucoma
    Bouhenni, Rachida A.
    Dunmire, Jeffrey
    Sewell, Abby
    Edward, Deepak P.
    [J]. JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, 2012,
  • [6] Mutant WDR36 directly affects axon growth of retinal ganglion cells leading to progressive retinal degeneration in mice
    Chi, Zai-Long
    Yasumoto, Fumie
    Sergeev, Yuri
    Minami, Masayoshi
    Obazawa, Minoru
    Kimura, Itaru
    Takada, Yuichiro
    Iwata, Takeshi
    [J]. HUMAN MOLECULAR GENETICS, 2010, 19 (19) : 3806 - 3815
  • [7] Optical coherence tomography using a frequency-tunable optical source
    Chinn, SR
    Swanson, EA
    Fujimoto, JG
    [J]. OPTICS LETTERS, 1997, 22 (05) : 340 - 342
  • [8] Sensitivity advantage of swept source and Fourier domain optical coherence tomography
    Choma, MA
    Sarunic, MV
    Yang, CH
    Izatt, JA
    [J]. OPTICS EXPRESS, 2003, 11 (18): : 2183 - 2189
  • [9] Application of a wide-field phantom eye for optical coherence tomography and reflectance imaging
    Corcoran, Anthony
    Muyo, Gonzalo
    van Hemert, Jano
    Gorman, Alistair
    Harvey, Andrew R.
    [J]. JOURNAL OF MODERN OPTICS, 2015, 62 (21) : 1828 - 1838
  • [10] Optical phantoms of varying geometry based on thin building blocks with controlled optical properties
    de Bruin, Daniel M.
    Bremmer, Rolf H.
    Kodach, Vitali M.
    de Kinkelder, Roy
    van Marle, Jan
    van Leeuwen, Ton G.
    Faber, Dirk J.
    [J]. JOURNAL OF BIOMEDICAL OPTICS, 2010, 15 (02)