Increased N250 elicited by facial familiarity: An ERP study including the face inversion effect and facial emotion processing

The present study aims to explore how familiarity modulates the neural processing of faces under different conditions: upright or inverted, neutral or emotional. To this purpose, 32 participants (25 female; age: M = 27.7 years, SD = 9.3) performed two face/emotion identification tasks during EEG recording. In the first task, to study facial processing, three different categories of facial stimuli were presented during a target detection task: famous familiar faces, faces of loved ones, and unfamiliar faces. To explore the face inversion effect according to each level of familiarity, these facial stimuli were also presented upside down. In the second task, to study emotional face processing, an emotional identification task on personally familiar and unfamiliar faces was conducted. The behavioural results showed an improved performance in the identification of facial expressions of emotion with the increase of facial familiarity, consistent with the previous literature. Regarding electrophysiological results, we found increased amplitudes of the P100, N170, and N250 for inverted compared to upright faces, independently of their degree of familiarity. Moreover, we did not find familiarity effects at the P100 and N170 timewindows, but we found that N250 amplitude was larger for personally familiar compared to unfamiliar faces. This result supports the reasoning that the facial familiarity increases the neural activity during the N250 timewindow, which may be explained by the processing of additional information prompted by the viewing of our loved ones faces, in contrast to what happens with unfamiliar individuals.