A new nano hyperbranched β-pinene polymer: Controlled synthesis and nonviral gene delivery

被引:5
作者
Rodrigues, Plinio R. [1 ,2 ]
Wang, Xianqing [2 ]
Li, Zishan [2 ]
Lyu, Jing [2 ]
Wang, Wenxin [2 ]
Vieira, Ronierik P. [1 ]
机构
[1] Univ Estadual Campinas, Sch Chem Engn, Dept Bioproc & Mat Engn, Albert Einstein St N 500,, BR-13083852 Campinas, SP, Brazil
[2] Univ Coll Dublin, Charles Inst Dermatol, Dublin 4, Ireland
关键词
Hyperbranched structure; DE-ATRP; Biocompatibility; DMAEMA; Gene transfection; ALPHA-PINENE; DE-ATRP;
D O I
10.1016/j.colsurfb.2022.113032
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Recently, an extensive research effort has been directed toward the improvement of nonviral transfection vectors, such as polymeric materials. The macromolecular structure of polymers has a substantial effect on their transfection efficacy. In this context, the modern advances in polymer production techniques, such as the deactivation-enhanced radical atom transfer polymerization (DE-ATRP), have been fundamental for the synthesis of controlled architecture nanomaterials. In this study, hyperbranched poly(beta-pinene)-PDMAEMA-PEGDMA nanometric copolymers were synthesised at high conversion via DE-ATRP using different concentrations of beta-pinene for gene delivery applications. The structural characterization and the biological performance of the materials were investigated. The copolymers' molar mass (10,434-16,438 mol l-1), dispersity, and conversion (90-95%) varied significantly with beta-pinene proportion on the polymerizations. The polymer-gene complexes generated (280-110 nm) presented excellent solution stability due to the beta-pinene segment on the copolymers. Gene delivery and transfection were highly dependent on the copolymer composition. The copolymers containing the highest beta-pinene proportions exhibited the best results with high transfection effectivity. In conclusion, the incorporation of beta-pinene in DMAEMA-PEGMA copolymer formulations is a renewable option to improve the materials' branching ratio, polyplex stability, and gene delivery performance without causing cytotoxic effects.
引用
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页数:9
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