Metabolomics and network pharmacology reveal the mechanism of Castanopsis honey against Streptococcus pyogenes

被引:11
作者
Yu, Wenjie [1 ]
Li, Xiaohua [2 ]
Sun, Qifang [2 ]
Yi, Shengxiang [2 ]
Zhang, Gaowei [2 ]
Chen, Lili [2 ]
Li, Zhuozhen [1 ]
Li, Junru [2 ]
Luo, Liping [1 ,2 ,3 ]
机构
[1] Beijing Technol & Business Univ, Sch Food & Hlth, Key Lab Geriatr Nutr & Hlth, Minist Educ, Beijing 100048, Peoples R China
[2] Nanchang Univ, Sch Life Sci, Sch Life Sci, Nanchang 330031, Peoples R China
[3] Nanchang Univ, State Key Lab Food Sci & Resources, Nanchang 330047, Peoples R China
关键词
Metabolomics; Network pharmacology; Honey; Streptococcus pyogenes; Antibacterial; Oxidative stress; ANTIBACTERIAL ACTIVITY; PERFORMANCE;
D O I
10.1016/j.foodchem.2024.138388
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Streptococcus pyogenes (GAS) is one of the most virulent and infectious bacteria, severely threatening health and lives of people worldwide. Honey has been proven to have effective capability against GAS, but the underlying metabolites and mechanisms are still unclear. In this study, the Castanopsis honey (CH) showed significant antibacterial ability compared to other seven kinds of honey and artificial honey. Furthermore, the antibacterial metabolites and their targets in CH were screened by combined method of metabolomics, network pharmacology, and molecular docking. The results suggested that the activities of two antioxidant enzymes, glutathione peroxidase and tyrosyl tRNA synthetase identified as the primary targets, were significantly inhibited by CH, which significantly increased the level of oxidative stress in GAS. The results revealed a possibly novel mechanism regulating the oxidative stress and inhibits the growth in bacteria, providing strong experimental evidence to support the further development of CH as a novel antibacterial agent.
引用
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页数:12
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