Type 2 diabetes mellitus stimulated pulmonary vascular inflammation and exosome biogenesis in rats

被引：3

作者：

Rezaie, Jafar ^{[1
]}

Hosseinpour, Hossein ^{[2
,3
]}

Rahbarghazi, Reza ^{[4
,5
]}

Keyhanmanesh, Rana ^{[4
]}

Khanzadeh, Shokoufeh ^{[2
]}

Mahdipour, Mahdi ^{[4
,5
]}

Soleimanpour, Jafar ^{[6
]}

Ahmadi, Mahdi ^{[7
]}

机构：

[1] Urmia Univ Med Sci, Solid Tumor Res Ctr, Cellular & Mol Med Inst, Orumiyeh, Iran

[2] Tabriz Univ Med Sci, Student Res Comm, Tabriz, Iran

[3] Tabriz Univ Med Sci, Fac Med, Dept Physiol, Tabriz, Iran

[4] Tabriz Univ Med Sci, Stem Cell Res Ctr, Tabriz, Iran

[5] Tabriz Univ Med Sci, Fac Adv Med Sci, Dept Appl Cell Sci, Tabriz, Iran

[6] Tabriz Univ Med Sci, Shohada Teaching Hosp, Dept Orthoped Surg, Tabriz, Iran

[7] Tabriz Univ Med Sci, Mol Med Res Ctr, Tabriz 5166616471, Iran

来源：

CELL BIOCHEMISTRY AND FUNCTION | 2023年 / 41卷 / 01期

关键词：

exosome biogenesis; inflammation; pulmonary tissue; rat; type; 2; diabetes; ADHESION MOLECULE-1 VCAM-1; CELL-ADHESION; COMPLICATIONS; EPIDEMIOLOGY; EXPRESSION;

D O I：

10.1002/cbf.3764

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It has been shown that type 2 Diabetes Mellitus (T2DM) changes the paracrine activity of several cell types. Whether the biogenesis of exosomes is changed during diabetic conditions is the subject of debate. Here, we investigated the effect of T2M on exosome biogenesis in rat pulmonary tissue. Rats received a high-fat diet regime and a single low dose of Streptozocin to mimic the T2DM-like condition. A total of 8 weeks after induction of T2DM, rats were subjected to several analyses. Besides histological examination, vascular cell adhesion molecule 1 (VCAM-1) levels were detected using immunohistochemistry (IHC) staining. Transcription of several genes such as IL-1 beta, Alix, and Rab27b was calculated by real-time polymerase chain reaction assay. Using western blot analysis, intracellular CD63 levels were measured. The morphology and exosome secretion activity were assessed using acetylcholinesterase (AChE) assay and scanning electron microscopy, respectively. Histological results exhibited a moderate-to-high rate of interstitial pneumonia with emphysematous changes. IHC staining showed an increased VCAM-1 expression in the diabetic lungs compared with the normal conditions (p < .05). Likewise, we found the induction of IL-1 beta, and exosome-related genes Alix and Rab27b under diabetic conditions compared with the control group (p < .05). Along with these changes, protein levels of CD63 and AChE activity were induced upon the initiation of T2DM, indicating accelerated exosome biogenesis. Taken together, current data indicated the induction of exosome biogenesis in rat pulmonary tissue affected by T2DM. It seems that the induction of inflammatory niche is touted as a stimulatory factor to accelerate exosome secretion.

引用

页码：78 / 85

页数：8

共 44 条

[1] Ageing and mesenchymal stem cells derived exosomes: Molecular insight and challenges [J].