Function Investigations and Applications of Membrane Proteins on Artificial Lipid Membranes

被引:14
|
作者
Tosaka, Toshiyuki [1 ]
Kamiya, Koki [1 ]
机构
[1] Gunma Univ, Div Mol Sci, Grad Sch Sci & Technol, Gunma 3768515, Japan
基金
日本学术振兴会;
关键词
membrane proteins; ion channels; nanopores; giant lipid vesicles; liposome; proteoliposome; planar bilayer lipid membrane; artificial cell model; GIANT UNILAMELLAR VESICLES; ION-CHANNEL PROTEINS; CELL-FREE SYNTHESIS; IN-VITRO; OUTER-MEMBRANE; PHYSIOLOGICAL CONDITIONS; LIPOSOME FORMATION; ESCHERICHIA-COLI; TRITON X-100; RECONSTITUTION;
D O I
10.3390/ijms24087231
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Membrane proteins play an important role in key cellular functions, such as signal transduction, apoptosis, and metabolism. Therefore, structural and functional studies of these proteins are essential in fields such as fundamental biology, medical science, pharmacology, biotechnology, and bioengineering. However, observing the precise elemental reactions and structures of membrane proteins is difficult, despite their functioning through interactions with various biomolecules in living cells. To investigate these properties, methodologies have been developed to study the functions of membrane proteins that have been purified from biological cells. In this paper, we introduce various methods for creating liposomes or lipid vesicles, from conventional to recent approaches, as well as techniques for reconstituting membrane proteins into artificial membranes. We also cover the different types of artificial membranes that can be used to observe the functions of reconstituted membrane proteins, including their structure, number of transmembrane domains, and functional type. Finally, we discuss the reconstitution of membrane proteins using a cell-free synthesis system and the reconstitution and function of multiple membrane proteins.
引用
收藏
页数:23
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