Angiogenic Potential of Plasma-Derived Extracellular Vesicles from Impaired Fasting Glucose Patients: A Pilot Study

被引：2

作者：

Castillo-Sanchez, Rocio ^{[1
]}

Candia-Plata, Maria ^{[1
]}

Ramirez-Romero, Astrid ^{[2
]}

Mata-Pineda, Ana ^{[1
]}

Martinez-Soto, Juan ^{[1
]}

Lopez-Soto, Luis ^{[1
]}

Galvan-Moroyoqui, Jose ^{[1
]}

Palomares, Ramon Iniguez ^{[3
]}

Rodriguez-Beas, Cesar ^{[3
]}

Alvarez-Ramos, Mario ^{[3
]}

Perez-Salazar, Eduardo ^{[4
]}

Soto-Guzman, Adriana ^{[1
]}

机构：

[1] Univ Sonora, Dept Med & Hlth Sci, Hermosillo, Sonora, Mexico

[2] Univ Sonora, Doctoral Program Chem & Hlth Sci, Hermosillo, Sonora, Mexico

[3] Univ Sonora, Dept Phys, Hermosillo, Sonora, Mexico

[4] IPN, Dept Cell Biol Cinvestav, Mexico City, DF, Mexico

来源：

BIOMEDICAL AND BIOTECHNOLOGY RESEARCH JOURNAL | 2023年 / 7卷 / 02期

关键词：

Angiogenesis; extracellular vesicles; impaired fasting glucose; migration; type; 2; diabetes; ENDOTHELIAL PROGENITOR CELLS; MICROVESICLES; TYPE-2; EXOSOMES; ENHANCE; CD105; VEGF;

D O I：

10.4103/bbrj.bbrj_56_23

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background: The goal of this study was to analyze the in vitro effect of plasma-isolated extracellular vesicles (EVs) from patients with impaired fasting glucose (IFG) on cell migration and angiogenic score on human endothelial cells (EA.hy926) cultures. Methods: Plasma samples from five patients with IFG, five with Type 2 diabetes mellitus (T2DM), and five normoglycemic subjects (controls) were used. Plasma-derived EVs were characterized by electron microscopy, dynamic light scattering and Western blotting, vascular endothelial growth factor receptor 2 (VEGFR-2), and endoglin detected in EVs by flow cytometry; wound closure assays and angiogenic score by matrigel assays in EA.hy926 cells were performed. Results: EA.hy926 cell migration induced by plasma-derived EVs from patients with IFG was greater than in control subjects (P = 0.023). EVs from patients with T2DM and IFG induced higher angiogenic scores than EVs from control subjects (P = 0.012 and P = 0.036, respectively). Conclusions: Endoglin and VEGFR-2 levels in EVs from IFG or T2DM patients were not different from those in control subjects. Plasma-derived EVs from patients with IFG and T2DM positively influenced human endothelial cell migration and angiogenic activity in vitro.

引用

页码：187 / 194

页数：8

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