Performance of Eversa Transform 2.0 Lipase in Ester Production Using Babassu Oil (Orbignya sp.) and Tucuman Oil (Astrocaryum vulgar): A Comparative Study between Liquid and Immobilized Forms in Fe3O4 Nanoparticles

被引：17

作者：

Brandao Junior, Joao ^{[1
]}

do Nascimento, Jean Gleison Andrade ^{[1
]}

Silva, Michael Pablo Franca ^{[1
]}

Brandao, Eliane de Aquino Lima ^{[2
]}

Bizerra, Viviane de Castro ^{[1
]}

dos Santos, Kaiany Moreira ^{[1
]}

Serpa, Juliana de Franca ^{[1
]}

dos Santos, Jose Cleiton Sousa ^{[1
]}

da Fonseca, Aluisio Marques ^{[1
]}

de Oliveira, Diego Lomonaco Vasconcelos ^{[3
]}

de Souza, Maria Cristiane Martins ^{[1
]}

机构：

[1] Univ Int Integrat Afro Brazilian Lusofonia UNILAB, Inst Engn & Sustainable Dev IEDS, Campus Auroras,Rua Jose Franco Oliveira,S-N Rural, BR-62790970 Redencao, CE, Brazil

[2] Fed Univ Rio Grande Norte UFRN, Technol Ctr, Chem Engn Postgrad Program, Campus Univ Lagoa Nova, BR-59078970 Natal, RN, Brazil

[3] Fed Univ Ceara UFC, Lab Prod & Technol Proc LPT, BR-60440900 Fortaleza, CE, Brazil

来源：

CATALYSTS | 2023年 / 13卷 / 03期

关键词：

biodiesel; babassu; tucuman; Eversa Transform 2.0; MAGNETIC NANOPARTICLES; BIODIESEL PRODUCTION; ENZYMATIC-SYNTHESIS; OPTIMIZATION; STRATEGIES; FAME;

D O I：

10.3390/catal13030571

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

In this study, biodiesel was produced through the enzymatic esterification of vegetable oils from two common Brazilian palm trees: babassu and tucuman. The oils were hydrolyzed by a chemical route and their free fatty acids esterified with ethanol and methanol using the lipase enzyme Eversa (R) Transform 2.0 in free forms and supported in iron magnetic nanoparticles (Fe3O4) (enzymatic load: 80 UpNPBg(-1)). These enzymatic reactions were performed at an oil-alcohol molar ratio of 1:1, reaction temperature of 37 degrees C, agitation at 150 rpm, and reaction times of 2, 4, 6 and 8 h for the reactions catalyzed by the soluble enzyme and 8 h for the reactions using the biocatalyst. The conversions of fatty acids in ethyl and methyl esters obtained were monitored by gas chromatography (CG). The results obtained from ester synthesis using enzyme catalysts in free form were better: babassu 52.6% (methanol) and 57.5% (ethanol), and for tucuman 96.7% (methanol) and 93.4% (ethanol). In the case of immobilized enzymes, the results obtained ranged from 68.7% to 82.2% for babassu and from 32.5% to 86.0% for tucuman, with three cycles of reuse and without significant catalyst loss. Molecular coupling studies revealed the structures of lipase and that linoleic acid bonded near the active site of the enzyme with the best free energy of -6.5 Kcal/mol.

引用

页数：19

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