Shoot organogenesis and plant regeneration from leaf and petiole explants of Corydalis saxicola Bunting

Corydalissaxicola Bunting, a medicinal herb known for alkaloids, grows mainly in Guizhou province and northwest Guangxi province of China. In this study, for the first time for this plant, an efficient adventitious shoot proliferation and regeneration system via shoot organogenesis from leaf and petiole explants was established. Cytokinins, including 6-benzyladenine (BA) and thidiazuron (TDZ), induced callus and then shoot organogenesis. Most adventitious shoots were induced from leaf explants on Murashige and Skoog (MS) medium (pH 6.5) supplemented with 2.0 mu M BA and 0.5 mu M alpha-naphthaleneacetic acid (NAA). This medium was also optimal for shoot proliferation. The rooting 1/2MS medium was supplemented with 1.0 mu M indole-3-butyric acid (IBA) and 0.2 mu M NAA; 95.7% of shoots induced roots within 30 d and 91% of plantlets survived on a peat:vermiculite (1:1, v:v) substrate after 90 d.