Do Circulating Extracellular Vesicles Strictly Reflect Bronchoalveolar Lavage Extracellular Vesicles in COPD?

被引：7

作者：

Tine, Mariaenrica ^{[1
]}

Neri, Tommaso ^{[2
]}

Biondini, Davide ^{[1
]}

Bernardinello, Nicol ^{[1
]}

Casara, Alvise ^{[1
]}

Conti, Maria ^{[1
]}

Minniti, Marianna ^{[2
]}

Cosio, Manuel G. ^{[1
,3
]}

Saetta, Marina ^{[1
]}

Celi, Alessandro ^{[2
]}

Nieri, Dario ^{[2
]}

Bazzan, Erica ^{[1
]}

机构：

[1] Univ Padua, Dept Cardiac Thorac Vasc Sci & Publ Hlth, I-35128 Padua, Italy

[2] Univ Pisa, Ctr Dipartimentale Biol Cellulare Cardiorespirato, Dipartimento Patol Chirurg Med Mol & Area Crit, I-56124 Pisa, Italy

[3] McGill Univ, Resp Div, Meakins Christie Labs, Montreal, PQ H3A 0G4, Canada

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2023年 / 24卷 / 03期

关键词：

COPD; extracellular vesicles; bronchoalveolar lavage; OBSTRUCTIVE PULMONARY-DISEASE; ENDOTHELIAL MICROPARTICLES; CIGARETTE-SMOKE; EXOSOMES; MICROVESICLES; DESTRUCTION; CELLS;

D O I：

10.3390/ijms24032966

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cell-derived extracellular vesicles (EVs) found in the circulation and body fluids contain biomolecules that could be used as biomarkers for lung and other diseases. EVs from bronchoalveolar lavage (BAL) might be more informative of lung abnormalities than EVs from blood, where information might be diluted. To compare EVs' characteristics in BAL and blood in smokers with and without COPD. Same-day BAL and blood samples were obtained in 9 nonsmokers (NS), 11 smokers w/o COPD (S), and 9 with COPD (SCOPD) (FEV1: 59 +/- 3% pred). After differential centrifugation, EVs (200-500 nm diameter) were identified by flow cytometry and labeled with cell-type specific antigens: CD14 for macrophage-derived EVs, CD326 for epithelial-derived EVs, CD146 for endothelial-derived EVs, and CD62E for activated-endothelial-derived EVs. In BAL, CD14-EVs were increased in S compared to NS [384 (56-567) vs. 172 (115-282) events/mu L; p = 0.007] and further increased in SCOPD [619 (224-888)] compared to both S (p = 0.04) and NS (p < 0.001). CD326-EVs were increased in S [760 (48-2856) events/mu L, p < 0.001] and in SCOPD [1055 (194-11,491), p < 0.001] when compared to NS [15 (0-68)]. CD146-EVs and CD62E-EVs were similar in the three groups. In BAL, significant differences in macrophage and epithelial-derived EVs can be clearly detected between NS, S and SCOPD, while these differences were not found in plasma. This suggests that BAL is a better medium than blood to study EVs in lung diseases.

引用

页数：11

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