An electrochemiluminescence biosensor based on Graphitic carbon nitride luminescence quenching for detection of AFB1

被引:40
|
作者
Tian, Dongyan [1 ]
Wang, Jie [1 ]
Zhuang, Qiandong [2 ]
Wu, Songmei [1 ]
Yu, Yu [1 ,3 ]
Ding, Kejian [1 ,3 ]
机构
[1] Beijing Jiaotong Univ, Sch Phys Sci & Engn, Beijing 100044, Peoples R China
[2] Univ Lancaster, Phys Dept, Lancaster LA1 4YB, England
[3] Beijing Jiaotong Univ, Sch Sci, Beijing 100044, Peoples R China
基金
中国国家自然科学基金;
关键词
Electrochemiluminesce biosensor; AflatoxinB1; Graphitic carbon nitride; Luminescence quenching; AFLATOXIN B-1;
D O I
10.1016/j.foodchem.2022.134183
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Based on graphite-like carbon nitride (g-CN) nanocomposites coupled with aptamer, a regenerable electro-chemiluminescence (ECL) biosensor is developed for the quantitative detection of aflatoxin B1 (AFB1). In the existence of AFB1, the structure of the aptamer changed into a loop, and the original ECL intensity was reduced owing to the enhancement of luminescence quenching between the ferrocene modified at the end of the aptamer and the luminescent substrate g-CN. Moreover, AFB1 with oxidation state could also react with high energy state g-CN, leading to further reduction of the electrochemiluminescence signal. At optimum conditions, ECL intensity was decreased in linearity with an AFB1 concentration range from 0.005 ng/mL to 10 ng/mL, and the minimum detectable concentration was down to 0.005 ng/mL, which realized trace detection demand with high sensitivity. It was selective for AFB1 and its performance had been verified on rice samples, which indicated a promising applying prospect of non-enzymatic electrochemiluminescence AFB1 detection.
引用
收藏
页数:7
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