In vivo imaging of the human retina using a two-photon excited fluorescence ophthalmoscope

被引：5

作者：

Boguslawski, Jakub ^{[1
,2
,3
]}

Tomczewski, Slawomir ^{[1
,2
]}

Dabrowski, Michal ^{[1
,2
]}

Komar, Katarzyna ^{[1
,2
,4
]}

Milkiewicz, Jadwiga ^{[1
,2
]}

Palczewska, Grazyna ^{[1
,2
,5
,6
]}

Palczewski, Krzysztof ^{[6
,7
,8
,9
]}

Wojtkowski, Maciej ^{[1
,2
]}

机构：

[1] Int Ctr Translat Eye Res, Skierniewicka 10A, PL-01230 Warsaw, Poland

[2] Polish Acad Sci, Inst Phys Chem, Kasprzaka 44-52, PL-01224 Warsaw, Poland

[3] Wroclaw Univ Sci & Technol, Fac Elect Photon & Microsyst, Laser & Fiber Elect Grp, Wybrze Wyspianskiego 27, PL-50370 Wroclaw, Poland

[4] Nicolaus Copernicus Univ Torun, Fac Phys Astron & Informat, Grudziadzka 5, PL-87100 Torun, Poland

[5] Polgenix Inc, Dept Med Devices, Cleveland, OH USA

[6] Univ Calif Irvine, Gavin Herbert Eye Inst, Dept Ophthalmol, Irvine, CA USA

[7] Univ Calif Irvine, Sch Med, Dept Physiol & Biophys, Irvine, CA USA

[8] Univ Calif Irvine, Dept Chem, Irvine, CA 92697 USA

[9] Univ Calif Irvine, Dept Mol Biol & Biochem, Irvine, CA 92697 USA

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 02期

关键词：

Bioinformatics; Biotechnology and Bioengineering; Clinical Protocol; Health Sciences; Molecular/Chemical Probes; Neuroscience; Physics;

D O I：

10.1016/j.xpro.2023.102225

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Noninvasive imaging of endogenous retinal fluorophores, including vitamin A derivatives, is vital to developing new treatments for retinal diseases. Here, we present a protocol for obtaining in vivo two-photon excited fluorescence images of the fundus in the human eye. We describe steps for laser characterization, sys-tem alignment, positioning human subjects, and data registration. We detail data processing and demonstrate analysis with example datasets. This technique al-lays safety concerns by allowing for the acquisition of informative images at low laser exposure. For complete details on the use and execution of this protocol, please refer to Boguslawski et al. (2022).1

引用

页数：20

共 9 条

[1] In vivo imaging of the human eye using a 2-photon-excited fluorescence scanning laser ophthalmoscope [J].

Boguslawski, Jakub ;

Palczewska, Grazyna ;

Tomczewski, Slawomir ;

Milkiewicz, Jadwiga ;

Kasprzycki, Piotr ;

Stachowiak, Dorota ;

Komar, Katarzyna ;

Marzejon, Marcin J. ;

Sikorski, Bartosz L. ;

Hudzikowski, Arkadiusz ;

Gluszek, Aleksander ;

Laszczych, Zbigniew ;

Karnowski, Karol ;

Sobon, Grzegorz ;

Palczewski, Krzysztof ;

Wojtkowski, Maciej .

JOURNAL OF CLINICAL INVESTIGATION, 2022, 132 (02)

[2] Optimal pupil size in the human eye for axial resolution [J].

Donnelly, WJ ;

Roorda, A .

JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 2003, 20 (11) :2010-2015

[3] GPScAN.VI: A general-purpose LabVIEW program for scanning imaging or any application requiring synchronous analog voltage generation and data acquisition [J].

Ferrand, Patrick .

COMPUTER PHYSICS COMMUNICATIONS, 2015, 192 :342-347

[4]

Guizar M, 2023, EFFICIENT SUBPIXEL I

[5] Efficient subpixel image registration algorithms [J].

Guizar-Sicairos, Manuel ;

Thurman, Samuel T. ;

Fienup, James R. .

OPTICS LETTERS, 2008, 33 (02) :156-158

[6] The empirical mode decomposition and the Hilbert spectrum for nonlinear and non-stationary time series analysis [J].

Huang, NE ;

Shen, Z ;

Long, SR ;

Wu, MLC ;

Shih, HH ;

Zheng, QN ;

Yen, NC ;

Tung, CC ;

Liu, HH .

PROCEEDINGS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES, 1998, 454 (1971) :903-995

[7] Noninvasive two-photon optical biopsy of retinal fluorophores [J].

Palczewska, Grazyna ;

Boguslawski, Jakub ;

Stremplewski, Patrycjusz ;

Kornaszewski, Lukasz ;

Zhang, Jianye ;

Dong, Zhiqian ;

Liang, Xiao-Xuan ;

Gratton, Enrico ;

Vogel, Alfred ;

Wojtkowski, Maciej ;

Palczewski, Krzysztof .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2020, 117 (36) :22532-22543

[8] Frequency-doubled femtosecond Er-doped fiber laser for two-photon excited fluorescence imaging [J].

Stachowiak, Dorota ;

Boguslawski, Jakub ;

Gluszek, Aleksander ;

Laszczych, Zbigniew ;

Wojtkowski, Maciej ;

Sobon, Grzegorz .

BIOMEDICAL OPTICS EXPRESS, 2020, 11 (08) :4431-4442

[9]

Wielgus M., 2012, 2012 15th International Conference on Information Fusion (FUSION 2012), P649

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