Intracellular chloride regulation mediates local sleep pressure in the cortex

被引：41

作者：

Alfonsa, Hannah ^{[1
]}

Burman, Richard J. ^{[1
]}

Brodersen, Paul J. N. ^{[1
]}

Newey, Sarah E. ^{[1
]}

Mahfooz, Kashif ^{[1
]}

Yamagata, Tomoko ^{[2
]}

Panayi, Marios C. ^{[3
]}

Bannerman, David M. ^{[3
]}

Vyazovskiy, Vladyslav V. ^{[2
]}

Akerman, Colin J. ^{[1
]}

机构：

[1] Univ Oxford, Dept Pharmacol, Oxford, England

[2] Univ Oxford, Dept Physiol Anat & Genet, Oxford, England

[3] Univ Oxford, Dept Expt Psychol, Oxford, England

来源：

NATURE NEUROSCIENCE | 2023年 / 26卷 / 01期

基金：

英国生物技术与生命科学研究理事会; 英国惠康基金; 欧洲研究理事会;

关键词：

EXCITATORY ACTIONS; EEG; GABA; DEPRIVATION; HOMEOSTASIS; INHIBITION; PLASTICITY; STIMULATION; RECOGNITION; MODULATION;

D O I：

10.1038/s41593-022-01214-2

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Extended wakefulness is associated with reduced performance and the build-up of sleep pressure. In the cortex, this manifests as changes in network activity. These changes show local variation depending on the waking experience, and their underlying mechanisms represent targets for overcoming the effects of tiredness. Here, we reveal a central role for intracellular chloride regulation, which sets the strength of postsynaptic inhibition via GABA(A) receptors in cortical pyramidal neurons. Wakefulness results in depolarizing shifts in the equilibrium potential for GABA(A) receptors, reflecting local activity-dependent processes during waking and involving changes in chloride cotransporter activity. These changes underlie electrophysiological and behavioral markers of local sleep pressure within the cortex, including the levels of slow-wave activity during non-rapid eye movement sleep and low-frequency oscillatory activity and reduced performance levels in the sleep-deprived awake state. These findings identify chloride regulation as a crucial link between sleep-wake history, cortical activity and behavior.

引用

页码：64 / +

页数：34

共 66 条

[1] The Contribution of Raised Intraneuronal Chloride to Epileptic Network Activity [J].