Development of a new plasma rich in growth factors membrane with improved optical properties

Purpose: The aim of the present work was to develop a fibrin membrane using plasma rich in growth factors (PRGF) technology with improved optical properties to be used for the treatment of ocular surface diseases. Basic procedures: Blood was drawn from three healthy donors, and the volume of PRGF obtained from each donor was divided into two main groups: i) PRGF or ii) platelet-poor plasma (PPP). Each membrane was then used pure or diluted to 90 %, 80 %, 70 %, 60 % and 50 %. The transparency of each of the different membranes was evaluated. The degradation and morphological characterization of each membrane was also performed. Finally, a stability study of the different fibrin membranes was performed.Main findings: The transmittance test showed that the fibrin membrane with the best optical characteristics was obtained after removal of platelets and dilution of fibrin to 50 % (50 % PPP). No significant differences (p > 0.05) were observed between the different membranes in the fibrin degradation test. The stability test showed that the membrane at 50 % PPP retains its optical and physical characteristics after storage at - 20 degrees C for 1 month compared to storage at 4 degrees C. Principalconclusions: The present study describes the development and characterization of a new fibrin membrane with improved optical characteristics while maintaining mechanical and biological character-istics. The physical and mechanical properties of the newly developed membrane are preserved after sto-rage for at least 1 month at - 20 degrees C.(c) 2023 Published by Elsevier GmbH.