Variability of 27 Autosomal STR Loci for the Population of the Republic of Belarus Based on the Mass Parallel Sequencing Data

被引：0

作者：

Kotova, S. A. ^{[1
]}

Parfionava, N. S. ^{[1
]}

Zabauskaya, T. V. ^{[1
]}

Rybakova, V. I. ^{[1
]}

Spivak, A. A. ^{[1
]}

Paliavoi, S. A. ^{[2
]}

Lugovnev, A. V. ^{[3
]}

机构：

[1] State Forens Examinat Comm Republ Belarus, Sci & Pract Ctr, Minsk 220114, BELARUS

[2] Masaryk Univ, Brno 60177, Czech Republic

[3] State Forens Examinat Comm Republ Belarus, Minsk 220073, BELARUS

来源：

RUSSIAN JOURNAL OF GENETICS | 2023年 / 59卷 / 03期

关键词：

autosomal STR locus; variability; isometric alleles; massively parallel sequencing; SYSTEM; IDENTIFICATION; DIVERSITY; MARKERS;

D O I：

10.1134/S1022795423030079

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The variability of 27 autosomal STR loci of the ForenSeq DNA Signature Prep Kit (Illumina) commercial panel was studied using the technique of massively parallel sequencing (MPS) in 733 unrelated individuals representing the population of the Republic of Belarus, as well as a population base of MPS allele frequencies for forensic probabilistic calculations in human identification and paternity testing was evaluated. The agreement between genotypes obtained by MPS and capillary electrophoresis (CE) was 99.96%. The number of MPS alleles increased more than two times for eight loci (D12S391, D21S11, D2S1338, vWA, D3S1358, D8S1179, D13S317, and D9S1122). Thirteen alleles detected were not included in the STRSeq BioProject catalog of the international online database STRbase 2.0. The random match probability of 27-locus MPS profiles decreased from 1.43 x 10(-31) to 2.89 x 10(-35), and the combined paternity index increased from 2.08 x 10(10) to 3.25 x 10(12) compared to CE data.

引用

页码：307 / 316

页数：10

共 24 条

[1] [Anonymous], 1982, Molecular Cloning: A Laboratory Manual
[2] biology-assets.anu.edu.au, GeneAlEx 6.5: Genetic Analysis in Excel
[3] Comparison of genomic DNA extraction techniques from whole blood samples: a time, cost and quality evaluation study
Chacon-Cortes, Diego
Haupt, Larisa M.
Lea, Rod A.
Griffiths, Lyn R.
[J]. MOLECULAR BIOLOGY REPORTS, 2012, 39 (05) : 5961 - 5966
[4] cmpg.unibe.ch, Arlequin: an integrated software for population genetics data analysis
[5] Committee on DNA Forensic Science, 1996, The Evaluation of Forensic DNA Evidence: an Update, DOI [10.17226/5141, DOI 10.17226/5141]
[6] High polymorphism detected by massively parallel sequencing of autosomal STRs using old blood samples from a Chinese Han population
Dai, Wenshen
Pan, Yajiao
Sun, Xiaochen
Wu, Riga
Li, Luo
Yang, Dongming
[J]. SCIENTIFIC REPORTS, 2019, 9 (1)
[7] Concordance of the ForenSeq™ system and characterisation of sequence-specific autosomal STR alleles across two major population groups
Devesse, Laurence
Ballard, David
Davenport, Lucinda
Riethorst, Immy
Mason-Buck, Gabriella
Court, Denise Syndercombe
[J]. FORENSIC SCIENCE INTERNATIONAL-GENETICS, 2018, 34 : 57 - 61
[8] Genome Reference Consortium, Human Build 37 patch release 13
[9] Sequence-based US population data for 27 autosomal STR loci
Gettings, Katherine Butler
Borsuk, Lisa A.
Steffen, Carolyn R.
Kiesler, Kevin M.
Vallone, Peter M.
[J]. FORENSIC SCIENCE INTERNATIONAL-GENETICS, 2018, 37 : 106 - 115
[10] STRSeq: A catalog of sequence diversity at human identification Short Tandem Repeat loci
Gettings, Katherine Butler
Borsuk, Lisa A.
Ballard, David
Bodner, Martin
Budowle, Bruce
Devesse, Laurence
King, Jonathan
Parson, Walther
Phillips, Christopher
Vallone, Peter M.
[J]. FORENSIC SCIENCE INTERNATIONAL-GENETICS, 2017, 31 : 111 - 117

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