piRNA-18 Inhibition Cell Proliferation, Migration and Invasion in Colorectal Cancer

被引：15

作者：

Liu, Qi ^{[1
,3
]}

Chen, Qian ^{[2
]}

Zhou, Zheng ^{[1
]}

Tian, Zeyu ^{[1
]}

Zheng, Ximin ^{[1
]}

Wang, Kaixuan ^{[1
]}

机构：

[1] Hunan Normal Univ, Affiliated Hosp 1, Hunan Prov Peoples Hosp, Dept Comprehens Surg, Changsha 410005, Hunan, Peoples R China

[2] Hunan Normal Univ, Affiliated Hosp 1, Hunan Prov Peoples Hosp, Dept Surg, Changsha 410005, Hunan, Peoples R China

[3] Hunan Emergency Ctr, 90 Pingchuan Rd, Changsha 410000, Hunan, Peoples R China

来源：

BIOCHEMICAL GENETICS | 2023年 / 61卷 / 05期

关键词：

Colorectal cancer; Short non-coding RNA; Piwi-RNA; Biological function; PIWI-INTERACTING RNAS; MIRNA EXPRESSION; DNA METHYLATION; GASTRIC-CANCER; BIOGENESIS; SIGNATURE; CARCINOMA; SURGERY; TARGET;

D O I：

10.1007/s10528-023-10348-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Colorectal cancer is one of the most prevalent malignancies worldwide. Evidences indicate that piRNA-18 are closely involved and contributed to tumorigenesis and cancer progression. Therefore, it is very necessary to investigate the effects of piRNA-18 on the proliferation, migration, and invasiveness of colorectal cancer cells, so as to provide theoretical basis for finding new biomarkers and accurate diagnosis and treatment of colorectal cancer. Here, Five pairs of colorectal cancer tissue samples and their corresponding adjacent samples were analyzed by real-time immunofluorescence quantitative PCR and the difference in piRNA-18 expression among colorectal cancer cell lines was further verified. MTT assay were used to study the changes in the proliferation of colorectal cancer cell lines after piRNA-18 overexpression. Wound-healing assay and Transwell assay were used to study the changes in migration and invasion. Flow cytometry were used to study the changes in apoptosis and cycle. SC inoculation of colorectal cancer cell lines into nude mice were used to observe the effect in the proliferation. piRNA-18 was lowlier expressed than adjacent tissues and normal intestinal mucosal epithelial cells in colorectal cancer and colorectal cancer cell line. After overexpression of piRNA-18, cell proliferation and migration as well as invasiveness in SW480 and LOVO cells decreased. The cell lines with piRNA-18 overexpression had obvious G1/S phase arrest in cell cycle, and the weight and volume of subcutaneously transplanted tumors are decreased. Our findings highlighted that piRNA-18 may play an inhibitory role in colorectal cancer.

引用

页码：1881 / 1897

页数：17