C2CD4B Evokes Oxidative Stress and Vascular Dysfunction via a PI3K/Akt/PKCα-Signaling Pathway

被引:4
作者
Di Pietro, Paola [1 ]
Abate, Angela Carmelita [1 ]
Prete, Valeria [1 ]
Damato, Antonio [2 ]
Venturini, Eleonora [2 ]
Rusciano, Maria Rosaria [1 ]
Izzo, Carmine [1 ]
Visco, Valeria [1 ]
Ciccarelli, Michele [1 ]
Vecchione, Carmine [1 ,2 ]
Carrizzo, Albino [1 ,2 ]
机构
[1] Univ Salerno, Scuola Med Salernitana, Dept Med Surg & Dent, I-84081 Baronissi, Italy
[2] IRCCS Neuromed, Vasc Physiopathol Unit, I-86077 Pozzilli, Italy
关键词
C2CD4B; endothelial dysfunction; oxidative stress; eNOS uncoupling; diabetes; PROTEIN-KINASE-C; NITRIC-OXIDE SYNTHASE; ENDOTHELIUM-DEPENDENT VASODILATION; GENOME-WIDE ASSOCIATION; BETA INHIBITOR; CELL; GLUCOSE; MECHANISMS; LOCI; RUBOXISTAURIN;
D O I
10.3390/antiox13010101
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
High glucose-induced endothelial dysfunction is an important pathological feature of diabetic vasculopathy. While genome-wide studies have identified an association between type 2 diabetes mellitus (T2DM) and increased expression of a C2 calcium-dependent domain containing 4B (C2CD4B), no study has yet explored the possible direct effect of C2CD4B on vascular function. Vascular reactivity studies were conducted using a pressure myograph, and nitric oxide and oxidative stress were assessed through difluorofluorescein diacetate and dihydroethidium, respectively. We demonstrate that high glucose upregulated both mRNA and protein expression of C2CD4B in mice mesenteric arteries in a time-dependent manner. Notably, the inhibition of C2CD4B expression by genetic knockdown efficiently prevented hyperglycemia-induced oxidative stress, endothelial dysfunction, and loss of nitric oxide (NO) bioavailability. Recombinant C2CD4B evoked endothelial dysfunction of mice mesenteric arteries, an effect associated with increased reactive oxygen species (ROS) and decreased NO production. In isolated human umbilical vein endothelial cells (HUVECs), C2CD4B increased phosphorylation of endothelial nitric oxide synthase (eNOS) at the inhibitory site Thr495 and reduced eNOS dimerization. Pharmacological inhibitors of phosphoinositide 3-kinase (PI3K), Akt, and PKC alpha effectively attenuated oxidative stress, NO reduction, impairment of endothelial function, and eNOS uncoupling induced by C2CD4B. These data demonstrate, for the first time, that C2CD4B exerts a direct effect on vascular endothelium via a phosphoinositide 3-kinase (PI3K)/Akt/PKC alpha-signaling pathway, providing a new perspective on C2CD4B as a promising therapeutic target for the prevention of oxidative stress in diabetes-induced endothelial dysfunction.
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页数:15
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