PARP1 Gene Knockout Suppresses Expression of DNA Base Excision Repair Genes

被引：1
|
作者：
Zakharenko, A. L. [1 ]
Malakhova, A. A. [1 ,2 ,3 ]
Dyrkheeva, N. S. [1 ]
Okorokova, L. S. [4 ]
Medvedev, S. P. [1 ,2 ,3 ]
Zakian, S. M. [1 ,2 ,3 ]
Kabilov, M. R. [1 ]
Tupikin, A. A. [1 ]
Lavrik, O. I. [1 ]
机构：
[1] Russian Acad Sci, Inst Chem Biol & Fundamental Med, Siberian Branch, Novosibirsk, Russia
[2] Russian Acad Sci, Inst Cytol & Genet, Fed Res Ctr, Siberian Branch, Novosibirsk, Russia
[3] Minist Hlth Russian Federat, Meshalkin Natl Med Res Ctr, Novosibirsk, Russia
[4] AcademGene LLC, Novosibirsk, Russia
来源：
DOKLADY BIOCHEMISTRY AND BIOPHYSICS | 2023年 / 508卷 / 01期
关键词：
PARP1; knockout; DEG; DNA Base Excision Repair; POLY(ADP-RIBOSE) POLYMERASE 1; FUNCTIONAL INTERACTION; GLYCOSYLASE NEIL1; ABASIC SITE; IDENTIFICATION; CLONING; GENOME;
D O I：
10.1134/S1607672922700028
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The effect of PARP1 knockout in HEK293 cells on the gene expression of DNA base excision repair (BER) proteins was studied. It was shown that the expression of all differentially expressed genes (DEGs) of BER was reduced by knockout. The expression of the DNA glycosylase gene NEIL1, which is considered to be one of the common "hubs" for binding BER proteins, has changed the most. The expression of genes of auxiliary subunits of DNA polymerases delta and epsilon is also significantly reduced. The PARP1 gene knockout cell line obtained is an adequate cell model for studying the activity of the BER process in the absence of PARP1 and testing drugs aimed at inhibiting repair processes. It has been found for the first time that knockout of the PARP1 gene results in a significant change in the level of expression of proteins responsible for ribosome biogenesis and the functioning of the proteasome.
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页码：6 / 11
页数：6
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