CD271 promotes proliferation and migration in bladder cancer

被引：1

作者：

Myoen, Shingo ^{[1
,2
,3
]}

Mochizuki, Mai ^{[1
]}

Shibuya-Takahashi, Rie ^{[1
]}

Fujimori, Haruna ^{[1
]}

Shindo, Norihisa ^{[4
]}

Yamaguchi, Kazunori ^{[4
]}

Yasuda, Jun ^{[4
]}

Abe, Jiro ^{[5
]}

Imai, Takayuki ^{[6
]}

Sato, Ikuro ^{[7
]}

Adachi, Hisanobu ^{[2
]}

Kawamura, Sadafumi ^{[2
]}

Ito, Akihiro ^{[3
]}

Tamai, Keiichi ^{[1
,8
]}

机构：

[1] Miyagi Canc Ctr, Res Inst, Div Canc Stem Cell, Natori, Miyagi, Japan

[2] Miyagi Canc Ctr, Div Urol, Natori, Miyagi, Japan

[3] Tohoku Univ, Grad Sch Med, Dept Urol, Sendai, Miyagi, Japan

[4] Miyagi Canc Ctr, Res Inst, Div Mol & Cellular Oncol, Natori, Miyagi, Japan

[5] Miyagi Canc Ctr, Div Thorac Surg, Natori, Miyagi, Japan

[6] Miyagi Canc Ctr, Div Head & Neck Surg, Natori, Miyagi, Japan

[7] Miyagi Canc Ctr, Div Pathol, Natori, Miyagi, Japan

[8] Miyagi Canc Ctr, Res Inst, Div Canc Stem Cell, 47-1 Medeshima Shiote, Natori, Miyagi 9811293, Japan

来源：

GENES TO CELLS | 2024年 / 29卷 / 01期

基金：

日本学术振兴会;

关键词：

bladder cancer; CD271; migration; proliferation; urothelial cancer; P75 NEUROTROPHIN RECEPTOR; EXPRESSION; RHO; ACTIVATION; PATHWAY; GROWTH;

D O I：

10.1111/gtc.13087

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Bladder cancer is a urothelial cancer and effective therapeutic strategies for its advanced stages are limited. Here, we report that CD271, a neurotrophin receptor, promotes the proliferation and migration of bladder cancer cells. CD271 knockdown decreased proliferation in both adherent and spheroid cultures, and vice versa when CD271 was overexpressed in bladder cancer cell lines. CD271 depletion impaired tumorigenicity in vivo. Migration activity was reduced by CD271 knockdown and TAT-Pep5, a known CD271-Rho GDI-binding inhibitor. Apoptosis was induced by CD271 knockdown. Comprehensive gene expression analysis revealed alterations in E2F- and Myc-related pathways upon CD271 expression. In clinical cases, patients with high CD271 expression showed significantly shortened overall survival. In surgically resected specimens, pERK, a known player in proliferation signaling, colocalizes with CD271. These data indicate that CD271 is involved in bladder cancer malignancy by promoting cell proliferation and migration, resulting in poor prognosis.

引用

页码：73 / 85

页数：13

共 40 条

[1]

[Anonymous], 2018, R LANG ENV STAT COMP

[2] Generation of Mice With a Conditional Allele for the p75NTR Neurotrophin Receptor Gene [J].

Bogenmann, Emil ;

Thomas, Penny S. ;

Li, Qianfeng ;

Kim, Jieun ;

Yang, Liang-Tung ;

Pierchala, Brian ;

Kaartinen, Vesa .

GENESIS, 2011, 49 (11) :862-869

[3] Human melanoma-initiating cells express neural crest nerve growth factor receptor CD271 [J].

Boiko, Alexander D. ;

Razorenova, Olga V. ;

van de Rijn, Matt ;

Swetter, Susan M. ;

Johnson, Denise L. ;

Ly, Daphne P. ;

Butler, Paris D. ;

Yang, George P. ;

Joshua, Benzion ;

Kaplan, Michael J. ;

Longaker, Michael T. ;

Weissman, Irving L. .

NATURE, 2010, 466 (7302) :133-U155

[4] p75(NTR): Receptor after all [J].

Bothwell, M .

SCIENCE, 1996, 272 (5261) :506-507

[5] Rho GTPases in cell biology [J].

Etienne-Manneville, S ;

Hall, A .

NATURE, 2002, 420 (6916) :629-635

[6] Establishment of a monoclonal antibody against glycosylated CD271 specific for cancer cells in immunohistochemistry [J].

Fujii, Keitaro ;

Morita, Shinkichi ;

Mochizuki, Mai ;

Shibuya-Takahashi, Rie ;

Fujimori, Haruna ;

Yamaguchi, Kazunori ;

Abe, Jiro ;

Yamazaki, Tomoko ;

Imai, Takayuki ;

Sugamura, Kazuo ;

Yasuda, Jun ;

Satoh, Kennichi ;

Sato, Ikuro ;

Saito-Koyama, Ryoko ;

Fujishima, Fumiyoshi ;

Sasano, Hironobu ;

Kato, Yukinari ;

Matsuura, Kazuto ;

Asada, Yukinori ;

Tamai, Keiichi .

CANCER SCIENCE, 2022, 113 (08) :2878-2887

[7] ERK/MAPK signalling pathway and tumorigenesis [J].

Guo, Yan-Jun ;

Pan, Wei-Wei ;

Liu, Sheng-Bing ;

Shen, Zhong-Fei ;

Xu, Ying ;

Hu, Ling-Ling .

EXPERIMENTAL AND THERAPEUTIC MEDICINE, 2020, 19 (03) :1997-2007

[8] CD271 Defines a Stem Cell-Like Population in Hypopharyngeal Cancer [J].

Imai, Takayuki ;

Tamai, Keiichi ;

Oizumi, Sayuri ;

Oyama, Kyoko ;

Yamaguchi, Kazunori ;

Sato, Ikuro ;

Satoh, Kennichi ;

Matsuura, Kazuto ;

Saijo, Shigeru ;

Sugamura, Kazuo ;

Tanaka, Nobuyuki .

PLOS ONE, 2013, 8 (04)

[9] Tumor-derived spheroids: Relevance to cancer stem cells and clinical applications [J].

Ishiguro, Tatsuya ;

Ohata, Hirokazu ;

Sato, Ai ;

Yamawaki, Kaoru ;

Enomoto, Takayuki ;

Okamoto, Koji .

CANCER SCIENCE, 2017, 108 (03) :283-289

[10] P75 neurotrophin receptor inhibits invasion and metastasis of gastric cancer [J].

Jin, Haifeng ;

Pan, Yanglin ;

He, Lijie ;

Zhai, Huihong ;

Li, Xiaohua ;

Zhao, Lina ;

Sun, Li ;

Liu, Jie ;

Hong, Liu ;

Song, Jiugang ;

Xie, Huahong ;

Gao, Juan ;

Han, Shuang ;

Li, Ying ;

Fan, Daiming .

MOLECULAR CANCER RESEARCH, 2007, 5 (05) :423-433

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