Mitochondrial DNA Corroborates the Genetic Variability of Clarias Catfishes (Siluriformes, Clariidae) from Cameroon

被引:0
作者
Kundu, Shantanu [1 ]
De Alwis, Piyumi S. [1 ]
Binarao, Jerome D. [1 ]
Lee, Soo-Rin [2 ]
Kim, Ah Ran [2 ]
Gietbong, Fantong Zealous [3 ]
Yi, Myunggi [4 ]
Kim, Hyun-Woo [1 ,2 ]
机构
[1] Pukyong Natl Univ, Dept Marine Biol, Busan 48513, South Korea
[2] Pukyong Natl Univ, Res Ctr Marine Integrated Technol Bion, Busan 48513, South Korea
[3] Minist Livestock Fisheries & Anim Ind MINEPIA, Yaounde, Cameroon
[4] Pukyong Natl Univ, Dept Biomed Engn, Busan 48513, South Korea
来源
LIFE-BASEL | 2023年 / 13卷 / 05期
基金
新加坡国家研究基金会;
关键词
walking catfish; DNA barcoding; haplotypes; phylogeny; genetic diversity; CLARIAS-GARIEPINUS BURCHELL; AFRICAN CATFISH; FISH; DIVERSITY; MACROCEPHALUS; CONSERVATION; POPULATIONS; TELEOSTEI; IDENTIFICATION;
D O I
10.3390/life13051068
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The airbreathing walking catfish (Clariidae: Clarias) comprises 32 species that are endemic to African freshwater systems. The species-level identification of this group is challenging due to their complex taxonomy and polymorphism. Prior to this study, the biological and ecological studies were restricted to a single species, Clarias gariepinus, resulting in a biased view of their genetic diversity in African waters. Here, we generated the 63-mitochondrial Cytochrome c oxidase subunit 1 (COI) gene sequences of Clarias camerunensis and Clarias gariepinus from the Nyong River in Cameroon. Both C. camerunensis and C. gariepinus species maintained adequate intra-species (2.7% and 2.31%) and inter-species (6.9% to 16.8% and 11.4% to 15.1%) genetic distances with other Clarias congeners distributed in African and Asian/Southeast Asian drainages. The mtCOI sequences revealed 13 and 20 unique haplotypes of C. camerunensis and C. gariepinus, respectively. The TCS networks revealed distinct haplotypes of C. camerunensis and shared haplotypes of C. gariepinus in African waters. The multiple species delimitation approaches (ABGD and PTP) revealed a total of 20 and 22 molecular operational taxonomic units (MOTUs), respectively. Among the two Clarias species examined, we found more than one MOTU in C. camerunensis, which is consistent with population structure and tree topology results. The phylogeny generated through Bayesian Inference analysis clearly separated C. camerunensis and C. gariepinus from other Clarias species with high posterior probability supports. The present study elucidates the occurrence of possible cryptic diversity and allopatric speciation of C. camerunensis in African drainages. Further, the present study confirms the reduced genetic diversity of C. gariepinus across its native and introduced range, which might have been induced by unscientific aquaculture practices. The study recommends a similar approach to the same and related species from different river basins to illuminate the true diversity of Clarias species in Africa and other countries.
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页数:12
相关论文
共 74 条
[1]   Insight into the phylogeny of African Clariidae (Teleostei, Siluriformes): Implications for their body shape evolution, biogeography, and taxonomy [J].
Agnese, JF ;
Teugels, GG .
MOLECULAR PHYLOGENETICS AND EVOLUTION, 2005, 36 (03) :546-553
[2]  
Awodiran M.O., 2018, FISH AQUA J, V9, P1000247, DOI [10.4172/2150-3508.1000247, DOI 10.4172/2150-3508.1000247]
[3]  
Baldwin CC, 2009, ZOOTAXA, P1
[4]   High genetic diversity and population differentiation in Clarias gariepinus of Yala Swamp: evidence from mitochondrial DNA sequencesa [J].
Barasa, J. E. ;
Abila, R. ;
Grobler, J. P. ;
Agaba, M. ;
Chemoiwa, E. J. ;
Kaunda-Arara, B. .
JOURNAL OF FISH BIOLOGY, 2016, 89 (06) :2557-2570
[5]   Genetic diversity and population structure of the African catfish, Clarias gariepinus (Burchell, 1822) in Kenya: implication for conservation and aquaculture [J].
Barasa, James E. ;
Mdyogolo, Sinebongo ;
Abila, Romulus ;
Grobler, Johannes Paul ;
Skilton, Robert A. ;
Bindeman, Hesmari ;
Njahira, Moses Ndotono ;
Chemoiwa, Emily J. ;
Dangasuk, Otto G. ;
Kaunda-Arara, Boaz ;
Verheyen, Erik .
BELGIAN JOURNAL OF ZOOLOGY, 2017, 147 (02) :105-127
[6]   Threats of global warming to the world's freshwater fishes [J].
Barbarossa, Valerio ;
Bosmans, Joyce ;
Wanders, Niko ;
King, Henry ;
Bierkens, Marc F. P. ;
Huijbregts, Mark A. J. ;
Schipper, Aafke M. .
NATURE COMMUNICATIONS, 2021, 12 (01)
[7]  
Bernt Maxwell J., 2022, American Museum Novitates, V3990, P1
[8]   Does fish ecology predict dispersal across a river drainage divide? [J].
Burridge, Christopher P. ;
Craw, Dave ;
Jack, Daniel C. ;
King, Tania M. ;
Waters, Jonathan M. .
EVOLUTION, 2008, 62 (06) :1484-1499
[9]   Assessment of Clarias gariepinus as a biological control agent against mosquito larvae [J].
Chala, Buze ;
Erko, Berhanu ;
Animut, Abebe ;
Degarege, Abraham ;
Petros, Beyene .
BMC ECOLOGY, 2016, 16
[10]   FUNDAMENTAL CONCEPTS IN GENETICS The genetics of inbreeding depression [J].
Charlesworth, Deborah ;
Willis, John H. .
NATURE REVIEWS GENETICS, 2009, 10 (11) :783-796