Robust Polyion Complex Vesicles (PICsomes) Based on PEO-b-poly(amino acid) Copolymers Combining Electrostatic and Hydrophobic Interactions: Formation, siRNA Loading and Intracellular Delivery

被引:12
作者
Aydinlioglu, Esra [1 ]
Abdelghani, Mona [2 ]
Le Fer, Gaelle [1 ,3 ]
van Hest, Jan C. M. [2 ]
Sandre, Olivier [1 ]
Lecommandoux, Sebastien [1 ]
机构
[1] Univ Bordeaux, CNRS, Bordeaux INP, UMR 5629,Lab Chim Polymeres Organ LCPO, ENSCBP 16 Ave Pey Berland, F-33607 Pessac, France
[2] Eindhoven Univ Technol, Inst Complex Mol Syst ICMS, Dept Biomed Engn, POB 513, NL-5600 MB Eindhoven, Netherlands
[3] Univ Lille, Un Materiaux & Transformat UMET, Ingenierie Syst Polymeres ISP Team, CNRS,INRAE,UMR 8270,Cent Lille, F-59000 Lille, France
关键词
PEO-b-poly(amino acids) block copolymers; polyion complexes; polypeptides; ring opening polymerization; small interfering RNA; CHARGED BLOCK-COPOLYMERS; ENHANCED STABILITY; PAIR; POLYMERSOMES; POLYPEPTIDE; NANOPARTICLES; PROGRESS; MICELLES; LAYER; SIZE;
D O I
10.1002/macp.202200306
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
Two pairs of oppositely charged PEO-b-poly(amino acid) copolymers with neutral poly(ethylene oxide) block and polypeptide block composed of the hydrophobic l-phenylalanine (Phe) amino acid mixed with either negative l-glutamic acid (Glu) or positive l-lysine (Lys) units are synthesized. N-carboxyanhydride (NCA) ring opening polymerization is performed with either PEO46-NH2 or PEO114-NH2 macroinitiators, leading respectively to PEO46-b-P(Glu(100)-co-Phe(65)) and PEO46-b-P(Lys(100)-co-Phe(65)), and PEO114-b-P(Glu(60)-co-Phe(40)) and PEO114-b-P(Lys(60)-co-Phe(40)). Polyion complexes (PIC) formed at near charge equilibrium led to vesicle formation (PICsomes), as shown by DLS, zetametry, and TEM. The good stability of PICsomes, even in high salinity media, is interpreted by pi-pi stacking hydrophobic interactions between the Phe residues, playing the role of "physical cross-linking". These PICsomes are successfully loaded with small interfering ribonucleic acid (siRNA) directed against firefly luciferase enzyme expression. They also exhibit minimal cell cytotoxicity while superior silencing efficacy is shown by cell bioluminescence assay as compared to free siRNA and a standard lipofectamine-siRNA complex. As such, self-assembly of oppositely charged PEO-b-poly(amino acids) block copolymers enables forming PICsomes of high stability thanks to pi-pi interactions of the Phe co-monomer in the polypeptide block, with high potential as biocompatible nanocarriers for RNA interference.
引用
收藏
页数:11
相关论文
共 58 条
[1]   Self-Assembly or Coassembly of Multiresponsive Histidine-Containing Elastin-Like Polypeptide Block Copolymers [J].
Abdelghani, Mona ;
Shao, Jingxin ;
Le, Duc H. T. ;
Wu, Hanglong ;
van Hest, Jan C. M. .
MACROMOLECULAR BIOSCIENCE, 2021, 21 (06)
[2]   Systemically Injectable Enzyme-Loaded Polyion Complex Vesicles as In Vivo Nanoreactors Functioning in Tumors [J].
Anraku, Yasutaka ;
Kishimura, Akihiro ;
Kamiya, Mako ;
Tanaka, Sayaka ;
Nomoto, Takahiro ;
Toh, Kazuko ;
Matsumoto, Yu ;
Fukushima, Shigeto ;
Sueyoshi, Daiki ;
Kano, Mitsunobu R. ;
Urano, Yasuteru ;
Nishiyama, Nobuhiro ;
Kataoka, Kazunori .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2016, 55 (02) :560-565
[3]   Living Unimodal Growth of Polyion Complex Vesicles via Two-Dimensional Supramolecular Polymerization [J].
Anraku, Yasutaka ;
Kishimura, Akihiro ;
Yamasaki, Yuichi ;
Kataoka, Kazunori .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2013, 135 (04) :1423-1429
[4]   Size-controlled long-circulating PICsome as a ruler to measure critical cut-off disposition size into normal and tumor tissues [J].
Anraku, Yasutaka ;
Kishimura, Akihiro ;
Kobayashi, Atsushi ;
Oba, Makoto ;
Kataoka, Kazunori .
CHEMICAL COMMUNICATIONS, 2011, 47 (21) :6054-6056
[5]   Spontaneous Formation of Nanosized Unilamellar Polyion Complex Vesicles with Tunable Size and Properties [J].
Anraku, Yasutaka ;
Kishimura, Akihiro ;
Oba, Makoto ;
Yamasaki, Yuichi ;
Kataoka, Kazunori .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2010, 132 (05) :1631-1636
[6]   MR imaging techniques for nano-pathophysiology and theranostics [J].
Bennett, Kevin M. ;
Jo, Jun-ichiro ;
Cabral, Horacio ;
Bakalova, Rumiana ;
Aoki, Ichio .
ADVANCED DRUG DELIVERY REVIEWS, 2014, 74 :75-94
[7]   Smart metallopoly(L-glutamic acid) polymers: reversible helix-to-coil transition at neutral pH [J].
Bonduelle, Colin ;
Makni, Fatma ;
Severac, Laura ;
Piedra-Arroni, Estefania ;
Serpentini, Charles-Louis ;
Lecommandoux, Sebastien ;
Pratviel, Genevieve .
RSC ADVANCES, 2016, 6 (88) :84694-84697
[8]   Tripartite siRNA micelles as controlled delivery systems for primary dendritic cells [J].
Boudier, Ariane ;
Aubert-Pouessel, Anne ;
Gerardin, Corine ;
Devoisselle, Jean-Marie ;
Begu, Sylvie ;
Louis-Plence, Pascale ;
Quentin, Julie ;
Jorgensen, Christian .
DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY, 2009, 35 (08) :950-958
[9]   Nucleic acids complexation with cationic elastin-like polypeptides: Stoichiometry and stability of nano-assemblies [J].
Bravo-Anaya, L. M. ;
Garbay, B. ;
Nando-Rodriguez, J. L. E. ;
Carvajal Ramos, F. ;
Ibarboure, E. ;
Bathany, K. ;
Xia, Y. ;
Rosselgong, J. ;
Joucla, G. ;
Garanger, E. ;
Lecommandoux, S. .
JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2019, 557 :777-792
[10]   Dynamic Stabilization of Unit Polyion Complexes Incorporating Small Interfering RNA by Fine-Tuning of Cationic Block Length in Two-Branched Poly(ethylene glycol)-b-poly(L-lysine) [J].
Chaya, Hiroyuki ;
Naito, Mitsuru ;
Cho, Masaru ;
Toh, Kazuko ;
Hayashi, Kotaro ;
Fukushima, Shigeto ;
Yamasaki, Yuichi ;
Kataoka, Kazunori ;
Miyata, Kanjiro .
BIOMACROMOLECULES, 2022, 23 (01) :388-397