Scarless genome editing technology and its application to crop improvement

被引：1
|
作者：
Ikeda, Kazuya [1 ]
机构：
[1] Bayspair Inc, 319 Bernardo Ave, Mountain View, CA 94043 USA
来源：
BREEDING SCIENCE | 2024年 / 74卷 / 01期
关键词：
genome editing; GWAS; scarless editing; WIDE ASSOCIATION; QUANTITATIVE RESISTANCE; LEAF-BLIGHT; PRECISE; BASE; CAS9; DNA; MECHANISMS; GENES; CELLS;
D O I：
10.1270/jsbbs.23045
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
The advent of CRISPR/Cas9 has had a disruptive impact on the world by bringing about dramatic progress and rapid penetration of genome editing technology. However, even though gene disruption can be easily achieved, there has been a challenge in freely changing the sequence. To solve this problem, various novel technologies have emerged in recent years to realize free rewriting of genome sequences. In this review, scarless editing by two-step HDR, a technology that can freely rewrite genomes from a single nucleotide to more than several thousand nucleotides, will be introduced.
引用
收藏
页码：32 / 36
页数：5
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