Polymorphism of SNP g.8398A>G at prolactin gene and its effect on Indonesian Holstein dairy cow's milk performance and reproductive traits

被引:0
作者
Setyorini, Y. W. [1 ,2 ]
Sutopo, S. [1 ]
Kurnianto, E. [1 ]
Sutiyono, S. [1 ]
机构
[1] Univ Diponegoro, Fac Anim & Agr Sci, Dept Anim Sci, Semarang 50275, Indonesia
[2] Minist Agr Indonesia, Balai Besar Pembibitan Ternak Unggul & Hijauan Pak, Banyumas 53151, Indonesia
关键词
Milk production; PCR-RFLP; Prolactin gene; Single nucleotide; ASSOCIATION; PRL; LOC514211; LOCUS;
D O I
10.14710/jitaa.48.1.10-19
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
The objective of this study was to identify the polymorphism of Single Nucleotide Polymorphism (SNP) g.8398A>G at the prolactin gene exon 4 in Indonesian Holstein dairy cows and its effect on milk production, milk quality, and reproductive traits. A total of 140 blood samples were investigated to detect polymorphism by PCR-RFLP method using the RsaI restriction enzyme. Sequencing was per-formed for confirmation of SNP mutation points. The phenotype data collected were milk production, milk quality, and reproductive traits. Data were analyzed using a t-test. The results showed that poly-morphism of SNP g.8398A>G at prolactin gene exon 4 was found in the study populations with AG and GG genotypes. Cows with the GG genotype indicated higher total milk yield and milk protein (P<0.05) than the AG genotype. Polymorphism was not associated with specific gravity, milk fat, milk lactose, total solid non-fat, and reproductive traits. SNP g.8398A>G at prolactin gene exon 4 showed potential as a genetic marker for selecting superior milk production traits.
引用
收藏
页码:10 / 19
页数:10
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