Insights into the reduction of antibiotic-resistant bacteria and mobile antibiotic resistance genes by black soldier fly larvae in chicken manure

被引:12
作者
Zhao, Zhengzheng [1 ,2 ]
Yang, Chongrui [1 ,2 ]
Gao, Bingqi [1 ,2 ]
Wu, Yushi [1 ,2 ]
Ao, Yue [3 ]
Ma, Shiteng [1 ,2 ]
Jimenez, Nuria [4 ]
Zheng, Longyu [1 ,2 ]
Huang, Feng [1 ,2 ]
Tomberlin, Jeffery K. [5 ]
Ren, Zhuqing [2 ,6 ]
Yu, Ziniu [1 ,2 ]
Yu, Chan [3 ]
Zhang, Jibin [1 ,2 ]
Cai, Minmin [1 ,2 ]
机构
[1] Huazhong Agr Univ, Coll Life Sci & Technol, Natl Engn Res Ctr Microbial Pesticides, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China
[2] Hubei Hongshan Lab, Wuhan 430070, Hubei, Peoples R China
[3] Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Peoples R China
[4] Univ Politecn Catalunya BarcelonaTech, Vilanova i la Geltru Sch Engn EPSEVG, Dept Chem Engn, Vilanova I La Geltru 08800, Spain
[5] Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA
[6] Huazhong Agr Univ, Coll Anim Sci, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ, Wuhan 430070, Hubei, Peoples R China
基金
中国国家自然科学基金;
关键词
Insect larvae; Antibiotic-resistant bacteria; Organic manure; Antimicrobial; Nutrition competition; FATE; INTEGRONS; EMISSION; PROTEIN; DAIRY;
D O I
10.1016/j.ecoenv.2023.115551
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The increasing prevalence of antibiotic-resistant bacteria (ARB) from animal manure has raised concerns about the potential threats to public health. The bioconversion of animal manure with insect larvae, such as the black soldier fly larvae (BSFL, Hermetia illucens [L.]), is a promising technology for quickly attenuating ARB while also recycling waste. In this study, we investigated BSFL conversion systems for chicken manure. Using metagenomic analysis, we tracked ARB and evaluated the resistome dissemination risk by investigating the co-occurrence of antibiotic resistance genes (ARGs), mobile genetic elements (MGEs), and bacterial taxa in a genetic context. Our results indicated that BSFL treatment effectively mitigated the relative abundance of ARB, ARGs, and MGEs by 34.9%, 53.3%, and 37.9%, respectively, within 28 days. Notably, the transferable ARGs decreased by 30.9%, indicating that BSFL treatment could mitigate the likelihood of ARG horizontal transfer and thus reduce the risk of ARB occurrence. In addition, the significantly positive correlation links between antimicrobial concentration and relative abundance of ARB reduced by 44.4%. Moreover, using variance partition analysis (VPA), we identified other bacteria as the most important factor influencing ARB, explaining 20.6% of the ARB patterns. Further analysis suggested that antagonism of other bacteria on ARB increased by 1.4 times, while nutrient competition on both total nitrogen and crude fat increased by 2.8 times. Overall, these findings provide insight into the mechanistic understanding of ARB reduction during BSFL treatment of chicken manure and provide a strategy for rapidly mitigating ARB in animal manure.
引用
收藏
页数:12
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