Proteome and phosphoproteome profiling of non-small cell lung cancer cell line A549 treated with TRAIL

被引：1

作者：

Zhong, Yi ^{[1
]}

Yang, Fen ^{[1
]}

Su, Tao ^{[1
]}

Wu, Xiyu ^{[1
]}

Zheng, Wen ^{[1
]}

Zhang, Lu ^{[1
]}

Liang, Ge ^{[1
]}

Wang, Lian ^{[2
]}

Wang, Lijun ^{[3
]}

Wang, Shisheng ^{[1
]}

Yang, Hao ^{[1
]}

机构：

[1] Sichuan Univ, West China Hosp, Regenerat Med Res Ctr, MOH,Key Lab Transplant Engn & Immunol,Prote Metab, Chengdu 610041, Peoples R China

[2] Chengdu Ctr Dis Control & Prevent, Chengdu, Peoples R China

[3] Southwest Jiaotong Univ, Affiliated Hosp, Peoples Hosp Chengdu 3, Dept Ophthalmol, Chengdu 610031, Peoples R China

来源：

PROTEOMICS | 2023年 / 23卷 / 3-4期

基金：

中国国家自然科学基金;

关键词：

DIA; phosphoproteome; proteome; TRAIL; APOPTOSIS-INDUCING LIGAND; DEATH; RECEPTOR; REPLICATION; MECHANISMS; EXPRESSION; AUTOPHAGY; DELIVERY; REVEALS; DAXX;

D O I：

10.1002/pmic.202200248

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is recognized for its promising therapeutic effects against cancer. However, mechanisms underlying the effect of TRAIL on protein expression, signal transduction, and apoptosis induction remain unclear. We surmised that a systematic analysis of the proteome and phosphoproteome associated with TRAIL signaling may help elucidate the mechanisms involved and facilitate the development of therapeutics. Therefore, we investigated the proteome and phosphoproteome of non-small cell lung cancer cell line A549 treated with TRAIL. Our results indicated that 126 proteins and 1684 phosphosites were markedly differentially expressed between the phosphate-buffered saline- and TRAIL-treated groups. The expression at protein and phosphosite levels were not completely consistent. Gene ontology functional analysis revealed that metal ion (zinc) binding was highly affected by TRAIL treatment. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis showed that almost all pathways that involved differentially expressed phosphosites were associated with apoptosis. We also identified an important kinase, AKT1, and its series of substrates in TRAIL signaling. The results of this study may provide guidance for future research on tumor therapy using TRAIL.

引用

页数：10

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