Comparative Structural Investigation of Histone-Like HU Proteins by Small-Angle X-ray Scattering

被引：0

作者：

Petoukhov, M. V. ^{[1
,2
,3
]}

Rakitina, T. V. ^{[2
,4
]}

Agapova, Yu. K. ^{[4
]}

Petrenko, D. E. ^{[4
]}

Konarev, P. V. ^{[1
,4
]}

Britikov, V. V. ^{[5
]}

Britikova, E. V. ^{[5
]}

Bocharov, E. V. ^{[2
]}

Shtykova, E. V. ^{[1
]}

机构：

[1] Russian Acad Sci, Shubnikov Inst Crystallog, Fed Sci Res Ctr Crystallog & Photon, Moscow 119333, Russia

[2] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow, Russia

[3] Russian Acad Sci, Frumkin Inst Phys Chem & Electrochem, Moscow 119071, Russia

[4] Natl Res Ctr, Kurchatov Inst, Moscow 123182, Russia

[5] Natl Acad Sci Belarus, Inst Bioorgan Chem, Minsk, BELARUS

来源：

CRYSTALLOGRAPHY REPORTS | 2023年 / 68卷 / 06期

基金：

俄罗斯科学基金会;

关键词：

BIOLOGICAL MACROMOLECULES; SPIROPLASMA-MELLIFERUM; THERMAL-STABILITY;

D O I：

10.1134/S1063774523600953

中图分类号：

O7 [晶体学];

学科分类号：

0702 ; 070205 ; 0703 ; 080501 ;

摘要：

Nucleoid-associated proteins (NAPs) control the structure and functions of bacterial nucleoid. Histone-like HU proteins are most abundant NAPs in dividing bacterial cells. Previously, structural ensembles of conformations of HU proteins from pathogenic mycoplasmas Spiroplasma melliferum and Mycoplasma gallisepticum were obtained using NMR spectroscopy. A structural study of these mycoplasma proteins is performed by small-angle X-ray scattering (SAXS). The occurrence of individual conformations from the ensemble, obtained by NMR, is estimated from the scattering data on HU protein solutions. In particular, an approach based on characterization of equilibrium mixtures in terms of volume fractions of their components was applied. The general shape of the proteins and their oligomeric state are independently confirmed using ab initio bead modelling. The flexibility of DNA-binding protein domains is analyzed by the ensemble optimization method, which is based on comparison of the structural characteristics of conformations fitting the SAXS data to the distribution of these characteristics in a randomly generated set. The results obtained give a new insight on the variability of the structure of HU proteins, which is necessary for their functioning.

引用

页码：912 / 919

页数：8

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