Ginkgo biloba supplement abates lead-induced endothelial and testicular dysfunction in Wistar rats via up-regulation of Bcl-2 protein expression, pituitary-testicular hormones and down-regulation of oxido-inflammatory reactions

被引：7

作者：

Asiwe, Jerome Ndudi ^{[1
,2
,3
]}

Ekene, Enekabokom Nwoke ^{[4
]}

Agbugba, Linda Chinyere ^{[5
]}

Moke, Emuesiri Goodies ^{[6
]}

Akintade, Abimbola Victoria ^{[7
]}

Ben-Azu, Benneth ^{[6
]}

Eruotor, Harrison ^{[8
]}

Daubry, Tarela Melish Elias ^{[3
]}

Anachuna, Kenneth Kelechi ^{[3
]}

Oyovwi, Mega O. ^{[9
]}

机构：

[1] PAMO Univ Med Sci, Fac Basic Med Sci, Dept Physiol, Port Harcourt, Nigeria

[2] Univ Ibadan, Fac Basic Med Sci, Dept Physiol, Ibadan, Nigeria

[3] Delta State Univ, Fac Basic Med Sci, Dept Physiol, Abraka, Nigeria

[4] Rivers State Univ, Fac Basic Clin Sci, Dept Pharmacol, Port Harcourt, Nigeria

[5] Univ Ibadan, Fac Vet Med, Dept Theriogenol, Ibadan, Nigeria

[6] Delta State Univ, Fac Basic Med Sci, Dept Pharmacol, Abraka, Nigeria

[7] Chrisland Univ, Fac Basic Med Sci, Dept Physiol, Abeokuta, Nigeria

[8] Univ Port Harcourt, Fac Basic Med Sci, Dept Biochem, Port Harcourt, Nigeria

[9] Achievers Univ, Fac Basic Med Sci, Dept Physiol, Owo, Ondo, Nigeria

来源：

JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY | 2023年 / 79卷

关键词：

Ginkgo biloba supplement; Endothelial dysfunction; Testicular dysfunction; Oxido-inflammatory stress; Lead acetate; Apoptosis; INDUCED OXIDATIVE STRESS; INDUCED LIVER-INJURY; NITRIC-OXIDE; SUPEROXIDE-DISMUTASE; SPERM MORPHOLOGY; PROTECTIVE ROLE; BLOOD-PRESSURE; EXPOSURE; EXTRACT; BILOBA;

D O I：

10.1016/j.jtemb.2023.127216

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: Apoptotic and oxido-inflammatory pathways have been found to be up-regulated in lead acetate poisoning which has been associated to endothelial and testicular dysfunctions. It is yet uncertain, nevertheless, if treatment with Ginkgo biloba supplements (GBS), a flavonoid-rich natural product can lessen the adverse effects of lead on endothelial and testicular functions. This study investigated the impact of Ginkgo biloba supplementation on lead-induced endothelial and testicular dysfunctions. Methods: The animals were treated with GBS (50 mg/kg and 100 mg/kg orally) for 14 days following oral exposure to lead acetate (25 mg/kg) for 14 days. After euthanasia, blood samples, epididymal sperm, testes, and aorta were collected. The quantities of the hormones (testosterone, follicle stimulating hormone (FSH) and luteinizing hormone (LH), as well as the anti-apoptotic, oxidative, nitrergic, inflammatory markers, were then determined using immunohistochemistry, ELISA, and conventional biochemical methods. Results: GBS reduced lead-induced oxidative stress by increasing the levels of the antioxidant enzymes catalase (CAT), glutathione (GSH), and superoxide dismutase (SOD), while lowering malondialdehyde (MDA) in endothelium and testicular cells. Normal testicular weight was restored by GBS which also decreased endothelial endothelin-I and increased nitrite levels. TNF-a and IL-6 were decreased while Bcl-2 protein expression was enhanced. Lead-induced alterations in reproductive hormones (FSH, LH, and testosterone) were also restored to normal. Conclusion: According to our result, using Ginkgo biloba supplement prevented lead from causing endothelial and testicular dysfunction by raising pituitary-testicular hormone levels, boosting Bcl-2 protein expression and lowering oxidative and inflammatory stress in the endothelium and testes.

引用

页数：14

共 99 条

[61] Molecular pathology of oxidative stress in diabetic angiopathy: Role of mitochondrial and cellular pathways [J].