Carbon dots promoted soybean photosynthesis and amino acid biosynthesis under drought stress: Reactive oxygen species scavenging and nitrogen metabolism

With global warming and water scarcity, improving the drought tolerance and quality of crops is critical for food se-curity and human health. Here, foliar application of carbon dots (CDs, 5 mg center dot L-1) could scavenge reactive oxygen spe-cies accumulation in soybean leaves under drought stress, thereby enhancing photosynthesis and carbohydrate transport. Moreover, CDs stimulated root secretion (e.g., amino acids, organic acids, and auxins) and recruited bene-ficial microorganisms (e.g., Actinobacteria, Ascomycota, Acidobacteria and Glomeromycota), which facilitate nitrogen (N) activation in the soil. Meanwhile, the expression of GmNRT, GmAMT, and GmAQP genes were up-regulated, indi-cating enhanced N and water uptake. The results demonstrated that CDs could promote nitrogen metabolism and enhance amino acid biosynthesis. Particularly, the N content in soybean shoots and roots increased significantly by 13.2 % and 30.5 %, respectively. The amino acids content in soybean shoots and roots increased by 257.5 % and 57.5 %, respectively. Consequently, soybean yields increased significantly by 21.5 %, and the protein content in soy-bean kernels improved by 3.7 %. Therefore, foliar application of CDs can support sustainable nano-enabled agriculture to combat climate change.