Photosynthetic co-culture system of algae and human umbilical vein endothelial cells: The effect on alleviating hypoxia and hypoxia/reoxygenation injury

被引:0
|
作者
Lin, Donghu [1 ,2 ]
Chen, Yuanyuan [3 ]
Tao, Xinyu [1 ,2 ]
Che, Xin [1 ]
Li, Shiyu [1 ,2 ]
Cheng, Shiyu [1 ,2 ]
Qu, Shuxin [1 ,2 ,4 ]
机构
[1] Southwest Jiaotong Univ, Inst Biomed Engn, Coll Med, Chengdu, Sichuan, Peoples R China
[2] Southwest Jiaotong Univ, Sch Mat Sci & Engn, Key Lab Adv Technol Mat, Minist Educ, Chengdu, Sichuan, Peoples R China
[3] Southwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu, Sichuan, Peoples R China
[4] Southwest Jiaotong Univ, Inst Biomed Engn, Coll Med, Chengdu 610031, Sichuan, Peoples R China
基金
中国国家自然科学基金;
关键词
biomaterials; biomedical application; bionic design; regenerative medicine; self-assembly; tissue engineering; OXYGEN; BIOMATERIALS; PHYSIOLOGY;
D O I
10.1049/bsb2.12078
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
It is a developed photosynthetic co-culture system to alleviate the hypoxia and hypoxia/reoxygenation (H/R)-injured human umbilical vein endothelial cells (HUVECs). The algae, Chlorella vulgaris, were encapsulated to slow their growth while not affecting the photosynthetic oxygen-producing capacity by Layer-by-layer (LbL) using gelatin and sodium alginate as the positive and negative charges materials, respectively. Then, the photosynthetic co-culture system of HUVECs and self-oxygenating alginate hydrogel (Algae-gel) was constructed in which the optimal ratios between algae and HUVECs were 5:1 and 20:1 for a 2D or 3D co-cultured manner, respectively. It indicated that the 3D co-cultured manner of HUVECs needed more O2 by the production of algae than it did in a 2D co-cultured manner. The co-cultured Algae-gel could alleviate hypoxia and the oxidative stress injury of hypoxia and hypoxia/reoxygenation (H/R)-treated HUVECs in the proliferation, intracellular ROS and cellular migratory ability. In addition, the Algae-gel could downregulate the expression of hypoxia-inducible factors 1 alpha (HIF-1 alpha) and vascular endothelial growth factor (VEGF) of hypoxia and H/R-injured HUVECs due to the improvement of hypoxia and H/R injury. This photosynthetic co-culture system could offer a promising approach for repairing hypoxia and H/R-injured cells or tissue by providing safe and stable O2.
引用
收藏
页码:76 / 88
页数:13
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