Optical trapping with holographically structured light for single-cell studies

被引：5

作者：

Lee, Moosung ^{[1
,2
]}

Hugonnet, Herve ^{[1
,2
]}

Lee, Mahn Jae ^{[2
,3
]}

Cho, Youngmoon ^{[1
,2
]}

Park, Yongkeun ^{[1
,2
,4
]}

机构：

[1] Korea Adv Inst Sci & Technol KAIST, Dept Phys, Daejeon 34141, South Korea

[2] KIHST, KAIST Inst Hlth Sci & Technol, Daejeon 34141, South Korea

[3] Korea Adv Inst Sci & Technol, Grad Sch Med Sci & Engn, Daejeon 34141, South Korea

[4] Tomocube Inc, Daejeon 34109, South Korea

来源：

BIOPHYSICS REVIEWS | 2023年 / 4卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

TOMOGRAPHIC DIFFRACTIVE MICROSCOPY; RED-BLOOD-CELLS; ANGULAR-MOMENTUM; NEURONAL GROWTH; FORCE; MANIPULATION; TWEEZERS; PHASE; FIBER; MICROMANIPULATION;

D O I：

10.1063/5.0111104

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A groundbreaking work in 1970 by Arthur Ashkin paved the way for developing various optical trapping techniques. Optical tweezers have become an established method for the manipulation of biological objects, due to their noninvasiveness and precise controllability. Recent innovations are accelerating and now enable single-cell manipulation through holographic light structuring. In this review, we provide an overview of recent advances in optical tweezer techniques for studies at the individual cell level. Our review focuses on holographic optical tweezers that utilize active spatial light modulators to noninvasively manipulate live cells. The versatility of the technology has led to valuable integrations with microscopy, microfluidics, and biotechnological techniques for various single-cell studies. We aim to recapitulate the basic principles of holographic optical tweezers, highlight trends in their biophysical applications, and discuss challenges and future prospects.

引用

页数：13

共 144 条

[1] Direct observation of base-pair stepping by RNA polymerase [J].