Identification of immune-responsive circular RNAs in shrimp (Litopenaeus vannamei) upon yellow head virus infection

被引:3
作者
Massu, Amarin [1 ]
Mahanil, Kanjana [1 ]
Limkul, Sirawich [1 ]
Phiwthong, Tannatorn [1 ]
Boonanuntanasarn, Surintorn [2 ]
Teaumroong, Neung [1 ]
Somboonwiwat, Kunlaya [3 ]
Boonchuen, Pakpoom [1 ]
机构
[1] Suranaree Univ Technol, Inst Agr Technol, Sch Biotechnol, Nakhon Ratchasima 30000, Thailand
[2] Suranaree Univ Technol, Sch Anim Technol & Innovat, Inst Agr Technol, Nakhon Ratchasima 30000, Thailand
[3] Chulalongkorn Univ, Fac Sci, Ctr Excellence Mol Biol & Genom Shrimp, Dept Biochem, Bangkok 10330, Thailand
关键词
Circular RNAs; Yellow head virus; circRNA sequencing; Litopenaeus vannamei; PENAEUS-MONODON; EXPRESSION; REGULATOR;
D O I
10.1016/j.fsi.2023.109246
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Circular RNAs (circRNAs) are a subclass of non-coding RNAs (ncRNAs) formed through a process known as back-splicing. They play a crucial role in the genetic regulation of various biological processes. Currently, circRNAs have been identified as participants in the antiviral response within mammalian cells. However, circRNAs in shrimp infected with the yellow head virus (YHV) remain largely unexplored. Therefore, this study aims to identify circRNAs in the hemocytes of Litopenaeus vannamei during YHV infection. We discovered 358 differ-entially expressed circRNAs (DECs), with 177 of them being up-regulated and 181 down-regulated. Subse-quently, eight DECs, including circ_alpha-1-inhibitor 3, circ_CDC42 small effector protein 2, circ_hemicentin 2, circ_integrin alpha V, circ_kazal-type proteinase inhibitor, circ_phenoloxidase 3, circ_related protein rab-8B, and circ_protein toll-like, were randomly selected for analysis of their expression patterns during YHV infection using qRT-PCR. Furthermore, the circRNAs' characteristics were confirmed through PCR, RNase R treatment, and Sanger sequencing, all of which were consistent with the features of circRNAs. These findings contribute to a better understanding of circRNAs' involvement in the antiviral response in shrimp.
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页数:10
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[31]   DISTRIBUTION OF YELLOW-HEAD VIRUS IN SELECTED TISSUES AND ORGANS OF PENAEID SHRIMP PENAEUS-VANNAMEI [J].
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