Principal component analysis-assisted zirconium-based metal-organic frameworks/DNA biosensor for the analysis of various phosphates

被引:3
作者
Teng, Qiuyi [1 ,2 ]
Zhou, Kunkun [1 ,2 ]
Yu, Kaihua [1 ,2 ]
Zhang, Xinyi [1 ,2 ]
Li, Zijun [1 ,2 ]
Wang, Huafeng [1 ,2 ]
Zhu, Chengzhi [1 ,2 ]
Wang, Zhaoyin [1 ,2 ]
Dai, Zhihui [1 ,2 ,3 ]
机构
[1] Nanjing Normal Univ, Jiangsu Collaborat Innovat Ctr Biomed Funct Mat, Nanjing 210023, Peoples R China
[2] Nanjing Normal Univ, Sch Chem & Mat Sci, Jiangsu Key Lab Biofunct Mat, Nanjing 210023, Peoples R China
[3] Nanjing Tech Univ, Sch Chem & Mol Engn, Nanjing 211816, Peoples R China
基金
中国国家自然科学基金;
关键词
Metal -organic frameworks; DNA; Phosphate; Principal component analysis; SIGNAL AMPLIFICATION; SENSITIVE DETECTION; SENSOR ARRAY; ATP;
D O I
10.1016/j.talanta.2024.125733
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Considering the diversity of phosphates and their pivotal roles in physiological processes, detection of various phosphates related to their metabolism is urgent but challenging. Herein, we design a biosensor with zirconiumbased MOFs (Zr-MOFs) and fluorophore-modified single-stranded DNA (F-ssDNA) for the analysis of phosphates. Relying on the interaction between Zr clusters and phosphate backbone, F-ssDNA is anchored on the surface of Zr-MOFs, inducing fluorescence resonance energy transfer (FRET) and subsequently quenching the fluorescence of F-ssDNA. Meanwhile, phosphates with different numbers of phosphate groups, molecular structures and coordination environments are able to adjust the FRET between Zr-MOFs and F-ssDNA via a site-occupying effect, recovering the fluorescence of F-ssDNA in distinct cases, which may result in diverse fluorescence signals. Consequently, seventeen phosphates and four phosphate mixtures are discriminated with the assistance of principal component analysis. These results provide new insight into the application of Zr-MOFs and broaden the path for the development of analytical methods for phosphates.
引用
收藏
页数:7
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共 45 条
  • [21] Microelectrode biosensor for real-time measurement of ATP in biological tissue
    Llaudet, E
    Hatz, S
    Droniou, M
    Dale, N
    [J]. ANALYTICAL CHEMISTRY, 2005, 77 (10) : 3267 - 3273
  • [22] Flavor components detection and discrimination of isomers in Huaguo tea using headspace-gas chromatography-ion mobility spectrometry and multivariate statistical analysis
    Lu, Wenhui
    Chen, Jing
    Li, Xuebo
    Qi, Yinghua
    Jiang, Rui
    [J]. ANALYTICA CHIMICA ACTA, 2023, 1243
  • [23] Linker-Eliminated Nano Metal-Organic Framework Fluorescent Probe for Highly Selective and Sensitive Phosphate Ratiometric Detection in Water and Body Fluids
    Ma, Yu
    Zhang, Yingqiu
    Li, Xiangyuan
    Yang, Peng
    Yue, Jie-Yu
    Jiang, Yu
    Tang, Bo
    [J]. ANALYTICAL CHEMISTRY, 2020, 92 (05) : 3722 - 3727
  • [24] Pillar[5]arene-Based Fluorescent Sensor Array for Biosensing of Intracellular Multi-neurotransmitters through Host-Guest Recognitions
    Mei, Yuxiao
    Zhang, Qi-Wei
    Gu, Qingyi
    Liu, Zhichao
    He, Xiao
    Tian, Yang
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2022, 144 (05) : 2351 - 2359
  • [25] Rare earth ions-enhanced gold nanoclusters as fluorescent sensor array for the detection and discrimination of phosphate anions
    Miao, Wenjing
    Wang, Lei
    Liu, Qin
    Guo, Shuai
    Zhao, Lingzhi
    Peng, Juanjuan
    [J]. CHEMISTRY-AN ASIAN JOURNAL, 2021, 16 (03) : 247 - 251
  • [26] Configurable fluorescent constructs for detection and discrimination of fluoride and biological phosphates
    Mohanty, Trupti
    Choudhury, Sharmistha Dutta
    [J]. JOURNAL OF MOLECULAR LIQUIDS, 2022, 358
  • [27] Europium oxide modified reduced graphene oxide composite for trace detection of hydrogen phosphate ions in soil samples
    Potdar, Revati P.
    Khollam, Yogesh B.
    Shaikh, Shoyebmohamad F.
    Patil, Supriya A.
    Al-Enizi, Abdullah M.
    More, Pravin S.
    [J]. TALANTA, 2024, 267
  • [28] A Review of MOFs and Their Composites-Based Photocatalysts: Synthesis and Applications
    Qian, Yongteng
    Zhang, Fangfang
    Pang, Huan
    [J]. ADVANCED FUNCTIONAL MATERIALS, 2021, 31 (37)
  • [29] Design and applications of metal-based molecular receptors and probes for inorganic phosphate
    Raju, Mandapati V. Ramakrishnam
    Harris, Sarah M.
    Pierre, Valerie C.
    [J]. CHEMICAL SOCIETY REVIEWS, 2020, 49 (04) : 1090 - 1108
  • [30] Tricolor emission carbon dots for label-free ratiometric fluorescent and colorimetric recognition of Al3+ and pyrophosphate ion and cellular imaging
    Shi, Lihong
    Wang, Qianliang
    Zhang, Caihong
    Zhang, Guomei
    Zhang, Yan
    Dong, Chuan
    Shuang, Shaomin
    [J]. SENSORS AND ACTUATORS B-CHEMICAL, 2021, 345