Protein film electrochemistry

被引:14
作者
Butt, Julea N. [1 ,2 ]
Jeuken, Lars J. C. [3 ]
Zhang, Huijie [3 ]
Burton, Joshua A. J. [1 ,2 ]
Sutton-Cook, Alexander L. [1 ,2 ]
机构
[1] Univ East Anglia, Sch Chem, Norwich, England
[2] Univ East Anglia, Sch Biol Sci, Norwich, England
[3] Leiden Univ, Leiden Inst Chem, Leiden, Netherlands
来源
NATURE REVIEWS METHODS PRIMERS | 2023年 / 3卷 / 01期
基金
英国生物技术与生命科学研究理事会;
关键词
DIRECT ELECTRON-TRANSFER; SELF-ASSEMBLED MONOLAYERS; C NITRITE REDUCTASE; CYTOCHROME-C; REDOX ENZYMES; IRON-SULFUR; FUMARATE REDUCTASE; CYCLIC VOLTAMMETRY; CARBON ELECTRODES; PROTON-TRANSFER;
D O I
10.1038/s43586-023-00262-7
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Protein film electrochemistry (PFE) is a powerful suite of electroanalytical techniques used to investigate the properties of redox proteins. The proteins under investigation are adsorbed as a (sub-)monolayer film on an electrode surface. Direct electron transfer between the immobilized protein and working electrode gives rise to an electrical current that visualizes and quantifies redox processes occurring within the protein. Advantages of PFE include low sample requirements - typically less than a nanomole protein - high sensitivity and the ability to resolve redox chemistry in the electrochemical potential and time domains. This Primer provides a guide to using PFE for quantitative thermodynamic and kinetic descriptions of half-reactions (redox reactions) and coupled chemical processes, including ligand binding, ligand unbinding and redox catalysis. Applications of PFE in developing biosensors, facilitating energy conversion and resolving enzyme mechanisms are highlighted. Finally, the state of the art and prospects for novel experimental and theoretical approaches are discussed. Protein film electrochemistry is used to study the redox properties of a protein adsorbed as a monolayer film on a working electrode. This Primer describes how protein film electrochemistry quantitatively investigates the thermodynamics and kinetics of processes such as ligand binding and redox catalysis.
引用
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页数:19
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