A mitochondria-targeted fluorescent probe for imaging of endogenous carbon monoxide in living cells

被引:9
作者
Yi, Mengwen [1 ,3 ]
Zhang, Nan [1 ]
Liu, Xiangjun [1 ,2 ]
Liu, Jing [1 ,2 ]
Zhang, Xiangru [1 ,2 ]
Wei, Yongbiao [3 ]
Shangguan, Dihua [1 ,2 ]
机构
[1] Chinese Acad Sci, Inst Chem, CAS Res Educ Ctr Excellence Mol Sci, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
[3] Guangxi Med Univ, Sch Pharmaceut Sci, Dept Pharmaceut Chem, 22, Shuangyong Rd, Nanning 530021, Guangxi, Peoples R China
基金
北京市自然科学基金; 中国国家自然科学基金;
关键词
Carbon monoxide; Fluorescent probe; Mitochondria; -targeted; Cell imaging; SENSITIVE DETECTION;
D O I
10.1016/j.saa.2023.122377
中图分类号
O433 [光谱学];
学科分类号
0703 ; 070302 ;
摘要
Carbon monoxide (CO), a vital gasotransmitter, plays critical functions in many physiological processes. Mitochondrial CO is closely related to mitochondrial respiration, thus the detection and imaging of mitochondrial CO in living cells is very important and has attracted much attention recently. In this paper, we developed a hemicyanine-based off-on fluorescent probe, CO-H1, which was used for monitoring endogenous mitochondrial CO levels in living cells. After reacted with CO in the presence of PdCl2, the fluorescence of CO-H1 was enhanced notably, accompanied by a significant red shift of absorption. CO-H1 exhibits low cytotoxicity, high sensitivity (detection limit of 0.048 mu M), and good selectivity for CO. When incubated with living cells, probe CO-H1 mainly entered the mitochondria. CO-H1 was successfully applied to imaging the exogenous/endogenous mitochondrial CO in living cells, suggesting its potential application for further studying the biological functions of mitochondrial CO in living cells.
引用
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页数:5
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