Generation of Patterned Cocultures in 2D and 3D: State of the Art

被引:3
作者
Joshi, Akshay [1 ]
Singh, Neetu [1 ,2 ]
机构
[1] Indian Inst Technol Delhi, Ctr Biomed Engn, Delhi 110016, India
[2] All India Inst Med Sci, Biomed Engn Unit, Delhi 110029, India
关键词
SMOOTH-MUSCLE-CELLS; MICROPATTERNED COCULTURE; ENDOTHELIAL-CELLS; FIBROUS MATS; SYSTEM; HEPATOCYTES; TISSUE; FIBROBLASTS; GROWTH; MODEL;
D O I
10.1021/acsomega.3c02713
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Cells inside the body are embedded into a highly structured microenvironment that consists of cells that lie in direct or close contact with other cell types that regulate the overall tissue function. Therefore, coculture models are versatile tools that can generate tissue engineering constructs with improved mimicking of in vivo conditions. While there are many reviews that have focused on pattering a single cell type, very few reviews have been focused on techniques for coculturing multiple cell types on a single substrate with precise control. In this regard, this Review covers various technologies that have been utilized for the development of these patterned coculture models while mentioning the limitations associated with each of them. Further, the application of these models to various tissue engineering applications has been discussed.
引用
收藏
页码:34249 / 34261
页数:13
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