CHARACTERIZATION OF THREE CITRULLUS LANATUS (THUNB.) MATSUM. & NAKAI VARIETIES BY FLUORESCENT CHROMOSOME STAINING AND RAPD ANALYSIS

被引:0
作者
Dash, Chandan kumar [1 ]
Afroz, Mahin [1 ,2 ]
Hossain, Afsana [1 ]
Sultana, Syeda sharmeen [1 ]
Das, Subroto kumar [1 ,2 ]
机构
[1] Univ Dhaka, Dept Bot, Cytogenet Lab, Dhaka 1000, Bangladesh
[2] Univ Barishal, Dept Bot, Barishal 8254, Bangladesh
来源
BANGLADESH JOURNAL OF BOTANY | 2023年 / 52卷 / 04期
关键词
Karyotype; CMA; DAPI; RAPD; Citrullus lanatus; WATERMELON CITRULLUS-LANATUS; GENETIC DIVERSITY;
D O I
10.3329/bjb.v52i4.70598
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Three commercial Citrullus lanatus varieties, namely Sonya, Kanya and Sweet black-2, were investigated with cyto-molecular techniques for characterization. Despite having similar somatic chromosome numbers and karyotype formulae (2n = 22), these three varieties had considerable diversities in Chromomycin A3 (CMA)-and 4 ', 6-diamidino-2-phenylindole (DAPI)-banding patterns. The total somatic chromosomal length was longest in Sweet black-2 (71.44 +/- 2.94 mu m) and shortest in Sonya (42.31 +/- 2.45 mu m). Each variety's unique GC-and AT-rich chromatin composition was represented by a variety of numbers, percentages, and patterns of distribution. Eleven random primers were employed to evaluate the genetic diversity among three varieties. Each variety showed a distinct fingerprinting pattern with 88.72% polymorphism while having 10 of the common RAPD bands.
引用
收藏
页码:1033 / 1040
页数:8
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