Rapid and sensitive detection of Mycoplasma synoviae using RPA combined with Pyrococcus furiosus Argonaute

被引：11
|
作者：
Zhao, Yanli [1 ,2 ]
Zhang, Yuhua [1 ,2 ]
Wu, Weiqing [1 ,2 ]
Kang, Tianhao [1 ,2 ]
Sun, Jian [1 ,2 ]
Jiang, Hongxia [1 ,2 ]
机构：
[1] South China Agr Univ, Coll Vet Med, Guangdong Key Lab Vet Pharmaceut Dev & Safety eval, Guangzhou 510642, Peoples R China
[2] South China Agr Univ, Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Peoples R China
来源：
POULTRY SCIENCE | 2024年 / 103卷 / 03期
关键词：
Mycoplasma synoviae; Pyrococcus furiosus Argonaute; recombinase polymerase; detection; AMPLIFICATION; PCR; GALLISEPTICUM; POULTRY; ASSAY;
D O I：
10.1016/j.psj.2023.103244
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
amplification and detection procedures can be completed within 40 min, whereas the results were superficially interpreted under UV and blue light. The sensitivity for MS detection was 2 copies/mL, and the specificity results showed no cross reaction with other pathogens. For the detection of 31 clinical samples, the results of this method and qPCR were completely consistent. This method provides a reliable and convenient method for the on-site detection of MS that is easy to operate without complex instruments and equipment. amplification and detection procedures can be completed within 40 min, whereas the results were superficially interpreted under UV and blue light. The sensitivity for MS detection was 2 copies/mL, and the specificity results showed no cross reaction with other pathogens. For the detection of 31 clinical samples, the results of this method and qPCR were completely consistent. This method provides a reliable and convenient method for the on-site detection of MS that is easy to operate without complex instruments and equipment.
引用
收藏
页数：9
相关论文
共 50 条
  • [1] Pyrococcus furiosus Argonaute Combined with Recombinase Polymerase Amplification for Rapid and Sensitive Detection of Enterocytozoon hepatopenaei
    Yang, Lihong
    Guo, Bo
    Wang, Yu
    Zhao, Chenjie
    Zhang, Xue
    Wang, Yue
    Tang, Yixin
    Shen, Hui
    Wang, Pei
    Gao, Song
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2023, 71 (01) : 944 - 951
  • [2] Rapid and Sensitive One-Tube Detection of Getah Virus Using RT-LAMP Combined with Pyrococcus furiosus Argonaute
    Liu, Zhong
    Yang, Fosheng
    Fang, Mengtao
    Wu, Qi
    Fan, Ke
    Huang, Dongyan
    Ye, Yu
    Wan, Gen
    Song, Deping
    VETERINARY SCIENCES, 2025, 12 (02)
  • [3] The detection of Salmonella in food based on PCR combined with Pyrococcus furiosus Argonaute
    Guo, Boyan
    Zhao, Yu
    Zhou, Changyu
    Li, Jinpeng
    Yang, Ming
    Li, Wenjing
    Xu, Qiang
    Zhou, Lin
    Lei, Changwei
    Wang, Hongning
    MICROCHEMICAL JOURNAL, 2024, 206
  • [4] Establishment of a simple, sensitive, and specific ASFV detection method based on Pyrococcus furiosus argonaute
    Zhao, Yu
    Yang, Ming
    Zhou, Changyu
    Guo, Boyan
    Wang, Kailu
    Song, Cailiang
    Wang, Hongning
    BIOSENSORS & BIOELECTRONICS, 2024, 254
  • [5] Using Recombinase-Aid Amplification Combined with Pyrococcus furiosus Argonaute for Rapid Sex Identification in Flamingo (Phoenicopteridae)
    Tan, Shenluan
    Zeng, Fanwen
    Zhong, Wanhuan
    Chen, Tanzipeng
    Chen, Xuanjiao
    Li, Li
    Wei, Hengxi
    Zhang, Shouquan
    ANIMALS, 2025, 15 (01):
  • [6] Pyrococcus furiosus Argonaute Based Detection Assays for Porcine Deltacoronavirus
    Zhao, Yu
    Zhang, Tiejun
    Zhou, Changyu
    Guo, Boyan
    Wang, Hongning
    ACS SYNTHETIC BIOLOGY, 2024, 13 (04): : 1323 - 1331
  • [7] Combination of ultrashort PCR and Pyrococcus furiosus Argonaute for DNA detection
    He, Ruyi
    Wang, Longyu
    Wang, Fei
    Yang, Jun
    Yu, Xiao
    Wang, Yuan
    Liu, Zhiguo
    Li, Chunhua
    Ma, Lixin
    ANALYST, 2021, 147 (01) : 35 - 39
  • [8] Pyrococcus furiosus Argonaute-mediated nucleic acid detection
    He, Ruyi
    Wang, Longyu
    Wang, Fei
    Li, Wenqiang
    Liu, Yang
    Li, Aitao
    Wang, Yuan
    Mao, Wuxiang
    Zhai, Chao
    Ma, Lixin
    CHEMICAL COMMUNICATIONS, 2019, 55 (88) : 13219 - 13222
  • [9] Pyrococcus furiosus argonaute based Alicyclobacillus acidoterrestrsis detection in fruit juice
    Shi, Yiheng
    Tan, Zishan
    Wu, Di
    Wu, Yongning
    Li, Guoliang
    FOOD MICROBIOLOGY, 2024, 120
  • [10] LAMP Assay Coupled with a Pyrococcus furiosus Argonaute System for the Rapid Detection of Porcine Epidemic Diarrhea Virus
    Yu, Zhaorong
    Shao, Ying
    Zhang, Yu
    Cheng, Fanyu
    Fang, Peng
    Tu, Jian
    Song, Xiangjun
    Qi, Kezong
    Wang, Zhenyu
    ACS SYNTHETIC BIOLOGY, 2025, 14 (03): : 689 - 698
12345