Toxic effects and potential mechanisms of zinc pyrithione (ZPT) exposure on sperm and testicular injury in zebrafish

被引:10
作者
Hu, Jinyuan [1 ]
Luo, Xu [1 ]
Panga, Mogellah John [1 ]
Appiah, Clara [1 ]
Retyunskiy, Vladimir [1 ]
Zhu, Lin [1 ]
Zhao, Ye [1 ]
机构
[1] Nanjing Tech Univ, Sch Pharmaceut Sci, Nanjing 211816, Peoples R China
基金
中国国家自然科学基金;
关键词
Zinc pyrithione (ZPT); Sperm; Testicular injury; RNA-seq; Zebrafish; ANTIFOULING BIOCIDES; BACTERIAL-INFECTION; OXIDATIVE STRESS; FATTY-ACIDS; APOPTOSIS; CELLS; PARAMETERS; EXPRESSION; FISH; MITOCHONDRIA;
D O I
10.1016/j.jhazmat.2023.132575
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Zinc pyrithione (ZPT) is widely recognized for its beneficial properties as an antifouling, antibacterial, and antifungal agent. Despite its positive industrial contributions, ZPT has been proven to exhibit toxicity towards various ecosystems, particularly affecting marine life. However, there is still a dearth of comprehensive research on ZPT toxicity and its toxicological mechanism in reproductive systems of aquatic organisms. In our study, we conducted a thorough analysis and unveiled a multitude of abnormalities in zebrafish sperm and testicular tissue caused by ZPT exposure, including a dose-dependent diminishing of testosterone levels, various sperm deformities, decreased sperm concentration and motility, and ROS-induced testicular tissue DNA damage. In addition, our study suggested that ZPT-induced testicular damage is associated with heightened oxidative stress, apoptosis, and possible hyperpolarization of the mitochondrial membrane. Through RNA-seq analysis, a total of 409 DEGs associated with ZPT-induced testicular injury were identified, and the hub gene was determined using a protein-protein interaction network (PPI). The genes and pathways uncovered in this study point to potential mechanisms of ZPT exposure on sperm and testicular injury in zebrafish.
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页数:16
相关论文
共 87 条
[1]   Mitochondrial membrane potential (MMP) regulates sperm motility [J].
Agnihotri, Saurabh Kumar ;
Agrawal, Ankit Kumar ;
Hakim, Bilal Ahmad ;
Vishwakarma, A. L. ;
Narender, T. ;
Sachan, Rekha ;
Sachdev, Monika .
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 2016, 52 (09) :953-960
[2]   In vivo exposure to codeine induces reproductive toxicity: role of HER2 and p53/Bcl-2 signaling pathway [J].
Ajayi, A. F. ;
Akhigbe, R. E. .
HELIYON, 2020, 6 (11)
[3]   Sodium arsenite accelerates D-galactose-induced aging in the testis of the rat: Evidence for mitochondrial oxidative damage, NF-kB, JNK, and apoptosis pathways [J].
Akbari, Sholeh ;
Amiri, Fereshteh Talebpour ;
Naderi, Maloos ;
Shaki, Fatemeh ;
Seyedabadi, Mohammad .
TOXICOLOGY, 2022, 470
[4]   Human ICE/CED-3 protease nomenclature [J].
Alnemri, ES ;
Livingston, DJ ;
Nicholson, DW ;
Salvesen, G ;
Thornberry, NA ;
Wong, WW ;
Yuan, JY .
CELL, 1996, 87 (02) :171-171
[5]   Thyroid hormone effects on androgen receptor messenger RNA expression in rat Sertoli and peritubular cells [J].
Arambepola, NK ;
Bunick, D ;
Cooke, PS .
JOURNAL OF ENDOCRINOLOGY, 1998, 156 (01) :43-50
[6]  
Bailey P., 2003, International Journal of Cosmetic Science, V25, P183, DOI 10.1046/j.1467-2494.2003.00183.x
[8]   Embryotoxicity of the antifouling biocide zinc pyrithione to sea urchin (Paracentrotus lividus) and mussel (Mytilus edulis) [J].
Bellas, J ;
Granmo, Å ;
Beiras, R .
MARINE POLLUTION BULLETIN, 2005, 50 (11) :1382-1385
[9]   Mitochondrial dysfunction and oxidative stress in metabolic disorders - A step towards mitochondria based therapeutic strategies [J].
Bhatti, Jasvinder Singh ;
Bhatti, Gurjit Kaur ;
Reddy, P. Hemachandra .
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, 2017, 1863 (05) :1066-1077
[10]   Improved method for the determination of zinc pyrithione in environmental water samples incorporating on-line extraction and preconcentration coupled with liquid chromatography atmospheric pressure chemical ionisation mass spectrometry [J].
Bones, Jonathan ;
Thomas, Kevin V. ;
Paull, Brett .
JOURNAL OF CHROMATOGRAPHY A, 2006, 1132 (1-2) :157-164