Development of a competitive ELISA based on estrogen receptor and weak competitive molecule for the screening of potential estrogens in foods

被引：18

作者：

Wang, Ying ^{[1
,2
]}

Wang, Minglu ^{[1
]}

Zhou, Li ^{[3
]}

Geng, Xiangye ^{[1
]}

Xu, Zhixiang ^{[2
]}

Zhang, Hongyan ^{[1
]}

机构：

[1] Shandong Normal Univ, Coll Life Sci, Shandong Prov Key Lab Anim Resistance Biol, Key Lab Food Nutr & Safety, Jinan 250014, Peoples R China

[2] Shandong Agr Univ, Coll Food Sci & Engn, Tai An 271018, Peoples R China

[3] Shandong Prov Inst Control Agrochem, Jinan 250131, Peoples R China

来源：

FOOD CHEMISTRY | 2023年 / 401卷

基金：

中国国家自然科学基金;

关键词：

Competitive ELISA; Weak competitive molecule; Estrogen receptor; Estrogen disrupting chemicals; Molecular docking; IN-VITRO; AFFINITY; MILK;

D O I：

10.1016/j.foodchem.2022.134084

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Enzyme labeled competitive molecules are generally homologous with competitors in competitive broad-spectrum enzyme-linked immunosorbent assays (ELISA). It is speculated that the detectability will be improved when the competitiveness of competitive molecule is weak. Herein, common small molecule food hazard-estrogen disrupting chemicals (EDCs) were used as target model for verification. The dual-estrogen receptor (ER) and three estrogen-enzyme conjugates with various responses were used as recognizers and competitive molecules in ELISA. ELISA based on bisphenol (BPA)-horseradish peroxidase (HRP) has the highest detectability and can screen all six EDCs, in which BPA-HRP showed the weakest ER excitatory activity (Ka = 1.39 x 10(-2) nmol center dot L-1) among three conjugates. The proposal showed good practicability with spiked recovery of 80.0-110 % for estrogens (17 beta-estradiol, 17 alpha-estradiol, BPA) in foodstuffs, and revealed biomarkers with weak competitiveness may be applied to other competitive procedures to improve detectability, and it provides sen-sitive pre-screening strategy for follow-up screening tool.

引用

页数：8

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