Antimicrobial activity of cinnamaldehyde against multidrug-resistant Klebsiella pneumoniae: an in vitro and in vivo study

被引：4
作者：
Mattos Vaz, Marcia Soares [1 ]
de Almeida de Souza, Gleyce Hellen [1 ]
dos Santos Radai, Joyce Alencar [1 ]
Fraga, Thiago Leite [2 ]
de Oliveira, Gustavo Gomes [2 ]
Wender, Heberton [3 ]
da Silva, Kesia Esther [1 ,4 ]
Simionatto, Simone [1 ]
机构：
[1] Univ Fed Grande Dourados UFGD, Lab Pesquisa Ciencias Saude, Itahum, Cidade Univ,Km 12, BR-79804970 Dourados, MS, Brazil
[2] Ctr Univ Grande Dourados UNIGRAN, Dourados, MS, Brazil
[3] Univ Fed Mato Grosso Do Sul, Inst Fis, Grp Pesquisa Nano & Fotons, Campo Grande, Brazil
[4] Stanford Univ, Div Infect Dis & Geog Med, Stanford, CA 94304 USA
来源：
BRAZILIAN JOURNAL OF MICROBIOLOGY | 2023年 / 54卷 / 03期
关键词：
Cinnamaldehyde; Multidrug-resistant; Gram-negative; Infection; ESCHERICHIA-COLI; INHIBITION; COMPONENTS; ACID;
D O I：
10.1007/s42770-023-01040-z
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The emergence and spread of multidrug-resistant (MDR) Klebsiella pneumoniae strains have increased worldwide, posing a significant health threat by limiting the therapeutic options. This study aimed to investigate the antimicrobial potential of cinnamaldehyde against MDR-K. pneumoniae strains in vitro and in vivo assays. The presence of resistant genes in MDR- K. pneumoniae strains were evaluated by Polymerase Chain Reaction (PCR) and DNA sequencing. Carbapenem-resistant K. pneumoniae strains show the bla(KPC-2) gene, while polymyxin-resistant K. pneumoniae presented bla(KPC-2) and alterations in the mgrB gene. Cinnamaldehyde exhibited an inhibitory effect against all MDR- K. pneumoniae evaluated. An infected mice model was used to determine the in vivo effects against two K. pneumoniae strains, one carbapenem-resistant and another polymyxin-resistant. After 24 h of cinnamaldehyde treatment, the bacterial load in blood and peritoneal fluids decreased. Cinnamaldehyde showed potential effectiveness as an antibacterial agent by inhibiting the growth of MDR-K. pneumoniae strains.
引用
收藏
页码：1655 / 1664
页数：10
相关论文
共 40 条
[21]   EXPERIMENTAL MODEL FOR TREATMENT OF EXTENDED SPECTRUM BETALACTAMASE PRODUCING-KLEBSIELLA PNEUMONIAE [J].
Michelon Toledo, Paula Virginia ;
Tuon, Felipe Francisco ;
Bail, Larissa ;
Manente, Francine ;
Arruda, Polliane ;
Aranha-Junior, Ayrton Alves .
ABCD-ARQUIVOS BRASILEIROS DE CIRURGIA DIGESTIVA-BRAZILIAN ARCHIVES OF DIGESTIVE SURGERY, 2014, 27 (03) :168-171
[22]   In Vitro and In Vivo Activities of OP0595, a New Diazabicyclooctane, against CTX-M-15-Positive Escherichia coli and KPC-Positive Klebsiella pneumoniae [J].
Morinaka, Akihiro ;
Tsutsumi, Yuko ;
Yamada, Keiko ;
Takayama, Yoshihiro ;
Sakakibara, Shiro ;
Takata, Toshihiko ;
Abe, Takao ;
Furuuchi, Takeshi ;
Inamura, Seiichi ;
Sakamaki, Yoshiaki ;
Tsujii, Nakako ;
Ida, Takashi .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2016, 60 (05) :3001-3006
[23]   Evaluation of essential oil obtained from Menthaxpiperita L. against multidrug-resistant strains [J].
Muntean, Delia ;
Licker, Monica ;
Alexa, Ersilia ;
Popescu, Iuliana ;
Jianu, Calin ;
Buda, Valentina ;
Dehelean, Cristina Adriana ;
Ghiulai, Roxana ;
Horhat, Florin ;
Horhat, Delia ;
Danciu, Corina .
INFECTION AND DRUG RESISTANCE, 2019, 12 :2905-2914
[24]   PROLONGED CHEMOKINE EXPRESSION AND EXCESSIVE NEUTROPHIL INFILTRATION IN THE LUNGS OF BURN-INJURED MICE EXPOSED TO ETHANOL AND PULMONARY INFECTION [J].
Murdoch, Eva L. ;
Karavitis, John ;
Deburghgraeve, Cory ;
Ramirez, Luis ;
Kovacs, Elizabeth J. .
SHOCK, 2011, 35 (04) :403-410
[25]   Klebsiella pneumoniae: a major worldwide source and shuttle for antibiotic resistance [J].
Navon-Venezia, Shiri ;
Kondratyeva, Kira ;
Carattoli, Alessandra .
FEMS MICROBIOLOGY REVIEWS, 2017, 41 (03) :252-275
[26]   PREVENTION OF DRUG ACCESS TO BACTERIAL TARGETS - PERMEABILITY BARRIERS AND ACTIVE EFFLUX [J].
NIKAIDO, H .
SCIENCE, 1994, 264 (5157) :382-388
[27]  
OECD O for EC and D, 2001, ENV HLTH SAFETY MONO
[28]   High Potency of Melaleuca alternifolia Essential Oil against Multi-Drug Resistant Gram-Negative Bacteria and Methicillin-Resistant Staphylococcus aureus [J].
Oliva, Alessandra ;
Costantini, Silvia ;
De Angelis, Massimiliano ;
Garzoli, Stefania ;
Bozovic, Mijat ;
Mascellino, Maria Teresa ;
Vullo, Vincenzo ;
Ragno, Rino .
MOLECULES, 2018, 23 (10)
[29]   In vitro antimicrobial activity and HPTLC analysis of hydroalcoholic seed extract of Nymphaea nouchali Burm. f. [J].
Parimala, Mabel ;
Shoba, Francis Gricilda .
BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2014, 14 :1-9
[30]   STUDIES ON TRANS-CINNAMALDEHYDE .1. THE INFLUENCE OF DOSE SIZE AND SEX ON ITS DISPOSITION IN THE RAT AND MOUSE [J].
PETERS, MMCG ;
CALDWELL, J .
FOOD AND CHEMICAL TOXICOLOGY, 1994, 32 (10) :869-876
1234