Antimicrobial activity of lipids extracted from Hermetia illucens reared on different substrates

被引：18

作者：

Franco, Antonio ^{[1
,2
]}

Scieuzo, Carmen ^{[1
,2
]}

Salvia, Rosanna ^{[1
,2
]}

Pucciarelli, Valentina ^{[1
]}

Borrelli, Luca ^{[3
]}

Addeo, Nicola Francesco ^{[3
]}

Bovera, Fulvia ^{[3
]}

Laginestra, Ambrogio ^{[4
]}

Schmitt, Eric ^{[5
]}

Falabella, Patrizia ^{[1
,2
]}

机构：

[1] Univ Basilicata, Dept Sci, Via Ateneo Lucano 10, I-85100 Potenza, Italy

[2] Univ Basilicata, Spinoff XFlies SRL, Via Ateneo Lucano 10, I-85100 Potenza, Italy

[3] Univ Naples Federico II, Dept Vet Med & Anim Prod, Via F Delpino 1, I-80137 Naples, Italy

[4] TotalEnergies EP Italia SPA, Dept Relat Terr, Via Tecn 4, I-85100 Potenza, Italy

[5] Protix BV, Ind Staat 3, NL-5107 NC Dongen, Netherlands

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2024年 / 108卷 / 01期

关键词：

Fatty acids; Antimicrobial resistance; Black soldier fly; Insects oil; BLACK SOLDIER FLY; FATTY-ACIDS; ORGANIC WASTE; LAURIC ACID; SALMONELLA; LARVAE; MEAL; ANTIBACTERIAL; COLONIZATION; PERFORMANCE;

D O I：

10.1007/s00253-024-13005-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

As the problem of antimicrobial resistance is constantly increasing, there is a renewed interest in antimicrobial products derived from natural sources, particularly obtained from innovative and eco-friendly materials. Insect lipids, due to their fatty acid composition, can be classified as natural antimicrobial compounds. In order to assess the antibacterial efficacy of Hermetia illucens lipids, we extracted this component from the larval stage, fed on different substrates and we characterized it. Moreover, we analyzed the fatty acid composition of the feeding substrate, to determine if and how it could affect the antimicrobial activity of the lipid component. The antimicrobial activity was evaluated against Gram-positive Micrococcus flavus and Gram-negative bacteria Escherichia coli. Analyzing the fatty acid profiles of larval lipids that showed activity against the two bacterial strains, we detected significant differences for C4:0, C10:0, C16:1, C18:3 n3 (ALA), and C20:1. The strongest antimicrobial activity was verified against Micrococcus flavus by lipids extracted from larvae reared on strawberry, tangerine, and fresh manure substrates, with growth inhibition zones ranged from 1.38 to 1.51 mm, while only the rearing on manure showed the effect against Escherichia coli. Notably, the fatty acid profile of H. illucens seems to not be really influenced by the substrate fatty acid profile, except for C18:0 and C18:2 CIS n6 (LA). This implies that other factors, such as the rearing conditions, larval development stages, and other nutrients such as carbohydrates, affect the amount of fatty acids in insects.

引用

页数：12